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Literature summary extracted from

  • Keller, M.; Dirmeier, R.
    Hydrogen-sulfur oxidoreductase complex from Pyrodictium abyssi (2001), Methods Enzymol., 331, 442-451.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.12.98.4 additional information no quinione, but enzyme complex contains cytochrome c and has two additional cytochromes, probably of b-type Pyrodictium abyssi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.4 Cu 1.2 mol per mol protein Pyrodictium abyssi
1.12.98.4 Fe 50-55 mol iron per mol protein Pyrodictium abyssi
1.12.98.4 Mo not detected Pyrodictium abyssi
1.12.98.4 Ni 1.6 mol per mol protein Pyrodictium abyssi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.4 52000
-
 enzym complex, gel-filtration Pyrodictium abyssi

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.4 Pyrodictium abyssi
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.98.4 the sulfur-reducing activity is irreversibly damaged by oxygen, anaerobic conditions: no loss of activity after one week, aerobic conditions: 80% loss of activity in 24 h Pyrodictium abyssi

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.4 enzyme complex Pyrodictium abyssi

Reaction

EC Number Reaction Comment Organism Reaction ID
1.12.98.4 H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1 a sulfur-reducing complex including hydrogenase, a sulfur reductase and electron-transferring components described Pyrodictium abyssi
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.4 7.56
-
 at 100°C Pyrodictium abyssi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.4 sulfur + electron donor electron donor: molecular hydrogen Pyrodictium abyssi H2S + oxidized electron donor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.98.4 More nine major polypeptides with molecular masses of 82000, 72000, 65000, 50000, 47000, 42000, 40000, 30000 and 24000 Da Pyrodictium abyssi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.98.4 100
-
 below 85°C about 10% loss of activity Pyrodictium abyssi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.98.4 8.5
-
-
 Pyrodictium abyssi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.12.98.4 7.5 9.5
-
 Pyrodictium abyssi