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Literature summary extracted from

  • Schröder, I.; Kröger, A.; Macy, J.M.
    Isolation of the sulphur reductase and reconstittutions of the sulphur respiration of Wolinella succinogenes (1988), Arch. Microbiol., 149, 572-579.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.12.98.4 additional information no lipophilic quinone, e.g. menaquinone (vitamin K2) involved, cytochrome b, c or covalently bound FAD are no cofactors Wolinella succinogenes

General Stability

EC Number General Stability Organism
1.12.98.4 anion exchange chromatography leads to severe loss of activity Wolinella succinogenes
1.12.98.4 gel filtration leads to severe loss of activity Wolinella succinogenes
1.12.98.4 sucrose density gradient centrifugation leads to severe loss of activity Wolinella succinogenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.98.4 cytoplasm
-
 Wolinella succinogenes 5737
-
1.12.98.4 membrane main activity membrane-bound Wolinella succinogenes 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.4 Fe iron-sulfur (non-heme) protein Wolinella succinogenes
1.12.98.4 Fe 1 or 2 FeS-clusters per subunit Wolinella succinogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.4 85000
-
 2 * 85000, SDS-PAGE Wolinella succinogenes
1.12.98.4 200000
-
 calculated from sedimentation coefficient obtained by sucrose density gradient centrifugation and Stokes radius obtained from gel filtration Wolinella succinogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.4 sulfur Wolinella succinogenes functional sulfur reductase that operates in the electron transport chain from fumarate to sulfur together with formate dehydrogenase, a membrane-bound and a cytoplasmic sulfur reductase H2S
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.12.98.4 Triton X-100 90% of the enzyme was solubilized Wolinella succinogenes

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.4 Wolinella succinogenes
-
 enzyme formed during growth at the expense of sulfur reduction by formate
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.4 isolation and incorporation into liposomes Wolinella succinogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.4 186
-
 sulfide oxidation Wolinella succinogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.4 sulfur functional sulfur reductase that operates in the electron transport chain from fumarate to sulfur together with formate dehydrogenase, a membrane-bound and a cytoplasmic sulfur reductase Wolinella succinogenes H2S
-
 ?
1.12.98.4 sulfur + electron donor electron donor: formate Wolinella succinogenes H2S + oxidized electron donor CO2 ?

Subunits

EC Number Subunits Comment Organism
1.12.98.4 dimer 2 * 85000, SDS-PAGE Wolinella succinogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12.98.4 additional information
-
 additional information liposomal preparation of membrane fraction: 2600 min-1, sulfide oxidation, liposomal preparation of enzyme: 1300 min-1, sulfide oxidation Wolinella succinogenes
1.12.98.4 additional information
-
 additional information liposomal preparation of enzyme : 2000 min-1 Wolinella succinogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.98.4 8.1
-
 sulfide oxidation Wolinella succinogenes