EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.10 | 1,10-phenanthroline | - |
Saccharomyces cerevisiae | |
1.5.1.10 | 2,2'-bipyridine | - |
Saccharomyces cerevisiae | |
1.5.1.10 | HgCl2 | - |
Saccharomyces cerevisiae | |
1.5.1.10 | additional information | not: carbonyl reagents | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.10 | 0.054 | - |
NAD+ | - |
Saccharomyces cerevisiae | |
1.5.1.10 | 2.32 | - |
L-saccharopine | - |
Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.10 | 50000 | - |
1 * 50000, SDS-PAGE | Saccharomyces cerevisiae |
1.5.1.10 | 67000 | - |
gel filtration with Sephadex G-100 | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.10 | 2-aminoadipate 6-semialdehyde + L-glutamate + NADPH | Saccharomyces cerevisiae | lysine biosynthesis | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.10 | Saccharomyces cerevisiae | - |
baker's yeast | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.5.1.10 | no glycoprotein | - |
Saccharomyces cerevisiae |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.10 | - |
Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.10 | 0.24 | - |
crude extract | Saccharomyces cerevisiae |
1.5.1.10 | 269.4 | - |
after purification | Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.10 | -70°C, pH 8.0, 10 mM 2-mercaptoethanol, 5 mM EDTA | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.10 | 2-aminoadipate 6-semialdehyde + L-glutamate + NADPH | - |
Saccharomyces cerevisiae | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | r | |
1.5.1.10 | 2-aminoadipate 6-semialdehyde + L-glutamate + NADPH | lysine biosynthesis | Saccharomyces cerevisiae | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
1.5.1.10 | N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | - |
Saccharomyces cerevisiae | L-glutamate + (S)-2-amino-6-oxohexanoate + NADH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.10 | monomer | 1 * 50000, SDS-PAGE | Saccharomyces cerevisiae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.10 | 34 | - |
denaturation above | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.10 | 9.5 | - |
saccharopine degradation | Saccharomyces cerevisiae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.10 | 9 | 10 | about 50% of activity maximum at pH 9 and 10 | Saccharomyces cerevisiae |