Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • von Schuhmann, G.; Gao, S.; Stöckigt, J.
    Vomilenine reductase - a novel enzyme catalyzing a crucial step in the biosynthesis of the therapeutically applied antiarrhythmic alkaloid ojmaline (2002), Bioorg. Med. Chem., 10, 1913-1928.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.32 EDTA 2 mM, 45% inhibition, complete inhibition after preincubation with 1 mM EDTA for 30 min, almost the total activity can be restored with 2 mM Co2+, Ni2+ and Mn2+ Rauvolfia serpentina
1.5.1.32 p-hydroxymercuribenzoate 0.2 mM, complete inhibition Rauvolfia serpentina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.32 additional information most probably a metalloprotein, complete inhibition after preincubation with 1 mM EDTA for 30 min, can be restored with 2 mM Co2+, Ni2+ and Mn2+ Rauvolfia serpentina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.32 43000
-
 gel filtration Rauvolfia serpentina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.32 vomilenine + NADPH Rauvolfia serpentina enzyme catalyzes a late step in the biosynthesis of the monoterpenoid indole alkaloid ajmaline 2-beta-(R)-1,2-dihydrovomilenine + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.32 Rauvolfia serpentina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.32 ammonium sulfate, Source 30Q, Source 15Phe, 2',5'-ADP-Sepharose Rauvolfia serpentina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.32 cell suspension culture
-
 Rauvolfia serpentina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.32 0.0031
-
-
 Rauvolfia serpentina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.32 vomilenine + NADPH
-
 Rauvolfia serpentina 2-beta-(R)-1,2-dihydrovomilenine + NADP+ non-natural 2alpha-(S)-1,2-dihydrovomilenine is never observed ?
1.5.1.32 vomilenine + NADPH enzyme catalyzes a late step in the biosynthesis of the monoterpenoid indole alkaloid ajmaline Rauvolfia serpentina 2-beta-(R)-1,2-dihydrovomilenine + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.32 monomer 1 * 43000, SDS-PAGE Rauvolfia serpentina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.32 30
-
 50% activity at 45°C Rauvolfia serpentina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.32 6
-
 in phosphate buffer, 50% activity at pH 5.2 and pH 6.8 respectively Rauvolfia serpentina
1.5.1.32 6.5
-
 in phosphate/citric acid buffer Rauvolfia serpentina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.32 5.2 6.8
-
 Rauvolfia serpentina

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.32 NADPH
-
 Rauvolfia serpentina