EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.20 | (+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | inhibits at high concentrations | Rattus norvegicus | |
1.5.1.20 | S-adenosylmethionine | in vivo feedback-inhibition | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.20 | 0.003 | - |
(+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Rattus norvegicus | |
1.5.1.20 | 0.023 | - |
5-methyltetrahydrofolate | - |
Rattus norvegicus | |
2.1.1.13 | 0.004 | - |
5-Methyl-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Rattus norvegicus | |
2.1.1.13 | 0.013 | - |
5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | - |
Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.20 | Rattus norvegicus | - |
adult male Sprague-Dawley rats, fed a Purina lab chow diet | - |
2.1.1.13 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.20 | more than 100fold partial purification | Rattus norvegicus |
2.1.1.13 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.20 | liver | - |
Rattus norvegicus | - |
2.1.1.13 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.20 | additional information | - |
specific activity is greater with the monoglutamate substrate than with the pentaglutamate | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.20 | 5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate + reduced acceptor | equilibrium lies far in favor of 5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate formation, pentaglutamate form binds to the same enzyme site as monoglutamate form | Rattus norvegicus | (+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate + oxidized acceptor | menadione as acceptor for 5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate formation | ? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: reduced acceptor is FADH2 | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | 5,10-methylene-5,6,7,8-tetrahydropteroylmonoglutamate as substrate | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | equilibrium lies far in favor of 5-methyltetrahydrofolate formation | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | reverse reaction: menadione as electron acceptor | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: NADH as reduced acceptor | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
1.5.1.20 | 5-methyltetrahydrofolate + NAD+ | - |
Rattus norvegicus | 5,10-methylenetetrahydrofolate + NADH + H+ | - |
r | |
1.5.1.20 | additional information | reverse reaction: not NADP+ as acceptor | Rattus norvegicus | ? | - |
? | |
2.1.1.13 | 5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate + L-homocysteine | - |
Rattus norvegicus | ? | - |
? | |
2.1.1.13 | N5-methyltetrahydrofolate + L-homocysteine | - |
Rattus norvegicus | tetrahydrofolate + L-methionine | - |
? | |
2.1.1.13 | N5-methyltetrahydropteroylmonoglutamate + L-homocysteine | - |
Rattus norvegicus | tetrahydropteroylmonoglutamate + L-methionine | - |
? | |
2.1.1.13 | N5-methyltetrahydropteroylpentaglutamate + L-homocysteine | - |
Rattus norvegicus | tetrahydropteroylpentaglutamate + L-methionine | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.20 | 30 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.20 | 6.7 | - |
5-methyltetrahydrofolate and 5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate as substrates | Rattus norvegicus |
2.1.1.13 | 6.5 | - |
- |
Rattus norvegicus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.20 | 6 | 8 | pH 6: about 70% of activity maximum, pH 8: about 35% of activity maximum with 5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate, about 65% of activity maximum with 5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.20 | FAD | flavoprotein, oxidized acceptor in reverse reaction | Rattus norvegicus | |
1.5.1.20 | FADH2 | flavoprotein, reduced acceptor in forward reaction | Rattus norvegicus | |
1.5.1.20 | NADH | - |
Rattus norvegicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.20 | 0.04 | 0.051 | (+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Rattus norvegicus |