BRENDA - Enzyme Database

Methionine biosynthesis (hog liver)

Buchanan, J.M.; Methods Enzymol. 17B, 371-378 (1971)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.1.1.13
acetone inactivates
Sus scrofa
2.1.1.13
freezing and thawing, 40% loss of activity
Sus scrofa
2.1.1.13
mercaptoethanol, 0.2 M, inactivation after 2 h at 24C or during freezing and thawing, no effect at 0C
Sus scrofa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.5.1.20
5,10-methylenetetrahydrofolate + FADH2
Escherichia coli
-
5-methyltetrahydrofolate + FAD
-
-
?
1.5.1.20
5,10-methylenetetrahydrofolate + FADH2
Sus scrofa
first step of conversion of 5,10-methylenetetrahydrofolate to methionine
5-methyltetrahydrofolate + FAD
-
-
?
1.5.1.20
5,10-methylenetetrahydrofolate + FADH2
Escherichia coli 113-3
-
5-methyltetrahydrofolate + FAD
-
-
?
2.1.1.13
5-methyltetrahydrofolate + L-homocysteine
Sus scrofa
methionine biosynthesis
tetrahydrofolate + L-methionine
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.5.1.20
Escherichia coli
-
-
-
1.5.1.20
Escherichia coli 113-3
-
-
-
1.5.1.20
Sus scrofa
-
-
-
2.1.1.13
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.5.1.20
-
Escherichia coli
1.5.1.20
20fold purification
Sus scrofa
2.1.1.13
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.5.1.20
liver
-
Sus scrofa
-
2.1.1.13
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.1.1.13
additional information
-
assay method
Sus scrofa
Storage Stability
EC Number
Storage Stability
Organism
1.5.1.20
-15C, several months, stable
Sus scrofa
2.1.1.13
0C, glutathione, N2-atmosphere, stable for at least a week
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.1.20
5,10-methylenetetrahydrofolate + FADH2
-
437712
Escherichia coli
5-methyltetrahydrofolate + FAD
-
-
-
?
1.5.1.20
5,10-methylenetetrahydrofolate + FADH2
first step of conversion of 5,10-methylenetetrahydrofolate to methionine
437712
Sus scrofa
5-methyltetrahydrofolate + FAD
-
-
-
?
1.5.1.20
5,10-methylenetetrahydrofolate + FADH2
-
437712
Escherichia coli 113-3
5-methyltetrahydrofolate + FAD
-
-
-
?
1.5.1.20
5,10-methylenetetrahydrofolate + reduced acceptor
forward reaction: reduced acceptor is FADH2
437712
Escherichia coli
5-methyltetrahydrofolate + oxidized acceptor
-
437712
Escherichia coli
r
1.5.1.20
5,10-methylenetetrahydrofolate + reduced acceptor
forward reaction: reduced acceptor is FADH2
437712
Sus scrofa
5-methyltetrahydrofolate + oxidized acceptor
-
437712
Sus scrofa
r
1.5.1.20
5,10-methylenetetrahydrofolate + reduced acceptor
reverse reaction: menadione as electron acceptor
437712
Escherichia coli
5-methyltetrahydrofolate + oxidized acceptor
-
437712
Escherichia coli
r
1.5.1.20
5,10-methylenetetrahydrofolate + reduced acceptor
reverse reaction: menadione as electron acceptor
437712
Sus scrofa
5-methyltetrahydrofolate + oxidized acceptor
-
437712
Sus scrofa
r
1.5.1.20
5,10-methylenetetrahydrofolate + reduced acceptor
forward reaction: reduced acceptor is FADH2
437712
Escherichia coli 113-3
5-methyltetrahydrofolate + oxidized acceptor
-
437712
Escherichia coli 113-3
r
1.5.1.20
5,10-methylenetetrahydrofolate + reduced acceptor
reverse reaction: menadione as electron acceptor
437712
Escherichia coli 113-3
5-methyltetrahydrofolate + oxidized acceptor
-
437712
Escherichia coli 113-3
r
2.1.1.13
5-methyltetrahydrofolate + L-homocysteine
methionine biosynthesis
437712
Sus scrofa
tetrahydrofolate + L-methionine
-
-
-
-
2.1.1.13
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
437712
Sus scrofa
tetrahydropteroylmonoglutamate + L-methionine
-
-
-
?
2.1.1.13
N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
437712
Sus scrofa
tetrahydropteroyltriglutamate + L-methionine
-
-
-
?
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.1.1.13
37
-
2 h, complete loss of activity
Sus scrofa
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.1.1.13
5
-
unstable
Sus scrofa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.13
S-adenosyl-L-methionine
required
Sus scrofa
2.1.1.13
vitamin B12
enzyme contains a derivative of vitamin B12 as prosthetic group
Sus scrofa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.13
S-adenosyl-L-methionine
required
Sus scrofa
2.1.1.13
vitamin B12
enzyme contains a derivative of vitamin B12 as prosthetic group
Sus scrofa
General Stability (protein specific)
EC Number
General Stability
Organism
2.1.1.13
acetone inactivates
Sus scrofa
2.1.1.13
freezing and thawing, 40% loss of activity
Sus scrofa
2.1.1.13
mercaptoethanol, 0.2 M, inactivation after 2 h at 24C or during freezing and thawing, no effect at 0C
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.5.1.20
5,10-methylenetetrahydrofolate + FADH2
Escherichia coli
-
5-methyltetrahydrofolate + FAD
-
-
?
1.5.1.20
5,10-methylenetetrahydrofolate + FADH2
Sus scrofa
first step of conversion of 5,10-methylenetetrahydrofolate to methionine
5-methyltetrahydrofolate + FAD
-
-
?
1.5.1.20
5,10-methylenetetrahydrofolate + FADH2
Escherichia coli 113-3
-
5-methyltetrahydrofolate + FAD
-
-
?
2.1.1.13
5-methyltetrahydrofolate + L-homocysteine
Sus scrofa
methionine biosynthesis
tetrahydrofolate + L-methionine
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.5.1.20
-
Escherichia coli
1.5.1.20
20fold purification
Sus scrofa
2.1.1.13
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.5.1.20
liver
-
Sus scrofa
-
2.1.1.13
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.1.1.13
additional information
-
assay method
Sus scrofa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.5.1.20
-15C, several months, stable
Sus scrofa
2.1.1.13
0C, glutathione, N2-atmosphere, stable for at least a week
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.1.20
5,10-methylenetetrahydrofolate + FADH2
-
437712
Escherichia coli
5-methyltetrahydrofolate + FAD
-
-
-
?
1.5.1.20
5,10-methylenetetrahydrofolate + FADH2
first step of conversion of 5,10-methylenetetrahydrofolate to methionine
437712
Sus scrofa
5-methyltetrahydrofolate + FAD
-
-
-
?
1.5.1.20
5,10-methylenetetrahydrofolate + FADH2
-
437712
Escherichia coli 113-3
5-methyltetrahydrofolate + FAD
-
-
-
?
1.5.1.20
5,10-methylenetetrahydrofolate + reduced acceptor
forward reaction: reduced acceptor is FADH2
437712
Escherichia coli
5-methyltetrahydrofolate + oxidized acceptor
-
437712
Escherichia coli
r
1.5.1.20
5,10-methylenetetrahydrofolate + reduced acceptor
forward reaction: reduced acceptor is FADH2
437712
Sus scrofa
5-methyltetrahydrofolate + oxidized acceptor
-
437712
Sus scrofa
r
1.5.1.20
5,10-methylenetetrahydrofolate + reduced acceptor
reverse reaction: menadione as electron acceptor
437712
Escherichia coli
5-methyltetrahydrofolate + oxidized acceptor
-
437712
Escherichia coli
r
1.5.1.20
5,10-methylenetetrahydrofolate + reduced acceptor
reverse reaction: menadione as electron acceptor
437712
Sus scrofa
5-methyltetrahydrofolate + oxidized acceptor
-
437712
Sus scrofa
r
1.5.1.20
5,10-methylenetetrahydrofolate + reduced acceptor
forward reaction: reduced acceptor is FADH2
437712
Escherichia coli 113-3
5-methyltetrahydrofolate + oxidized acceptor
-
437712
Escherichia coli 113-3
r
1.5.1.20
5,10-methylenetetrahydrofolate + reduced acceptor
reverse reaction: menadione as electron acceptor
437712
Escherichia coli 113-3
5-methyltetrahydrofolate + oxidized acceptor
-
437712
Escherichia coli 113-3
r
2.1.1.13
5-methyltetrahydrofolate + L-homocysteine
methionine biosynthesis
437712
Sus scrofa
tetrahydrofolate + L-methionine
-
-
-
-
2.1.1.13
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
437712
Sus scrofa
tetrahydropteroylmonoglutamate + L-methionine
-
-
-
?
2.1.1.13
N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
437712
Sus scrofa
tetrahydropteroyltriglutamate + L-methionine
-
-
-
?
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.1.1.13
37
-
2 h, complete loss of activity
Sus scrofa
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.1.1.13
5
-
unstable
Sus scrofa