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Literature summary extracted from

  • Buchanan, J.M.
    Methionine biosynthesis (hog liver) (1971), Methods Enzymol., 17B, 371-378.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.1.1.13 acetone inactivates Sus scrofa
2.1.1.13 freezing and thawing, 40% loss of activity Sus scrofa
2.1.1.13 mercaptoethanol, 0.2 M, inactivation after 2 h at 24°C or during freezing and thawing, no effect at 0°C Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.20 5,10-methylenetetrahydrofolate + FADH2 Escherichia coli
-
 5-methyltetrahydrofolate + FAD
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + FADH2 Sus scrofa first step of conversion of 5,10-methylenetetrahydrofolate to methionine 5-methyltetrahydrofolate + FAD
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + FADH2 Escherichia coli 113-3
-
 5-methyltetrahydrofolate + FAD
-
 ?
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine Sus scrofa methionine biosynthesis tetrahydrofolate + L-methionine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.20 Escherichia coli
-
-
-
1.5.1.20 Escherichia coli 113-3
-
-
-
1.5.1.20 Sus scrofa
-
-
-
2.1.1.13 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.20
-
 Escherichia coli
1.5.1.20 20fold purification Sus scrofa
2.1.1.13
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.20 liver
-
 Sus scrofa
-
2.1.1.13 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.13 additional information
-
 assay method Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.5.1.20 -15°C, several months, stable Sus scrofa
2.1.1.13 0°C, glutathione, N2-atmosphere, stable for at least a week Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.20 5,10-methylenetetrahydrofolate + FADH2
-
 Escherichia coli 5-methyltetrahydrofolate + FAD
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + FADH2 first step of conversion of 5,10-methylenetetrahydrofolate to methionine Sus scrofa 5-methyltetrahydrofolate + FAD
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + FADH2
-
 Escherichia coli 113-3 5-methyltetrahydrofolate + FAD
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor forward reaction: reduced acceptor is FADH2 Escherichia coli 5-methyltetrahydrofolate + oxidized acceptor
-
 r
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor forward reaction: reduced acceptor is FADH2 Sus scrofa 5-methyltetrahydrofolate + oxidized acceptor
-
 r
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor reverse reaction: menadione as electron acceptor Escherichia coli 5-methyltetrahydrofolate + oxidized acceptor
-
 r
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor reverse reaction: menadione as electron acceptor Sus scrofa 5-methyltetrahydrofolate + oxidized acceptor
-
 r
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor forward reaction: reduced acceptor is FADH2 Escherichia coli 113-3 5-methyltetrahydrofolate + oxidized acceptor
-
 r
1.5.1.20 5,10-methylenetetrahydrofolate + reduced acceptor reverse reaction: menadione as electron acceptor Escherichia coli 113-3 5-methyltetrahydrofolate + oxidized acceptor
-
 r
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine methionine biosynthesis Sus scrofa tetrahydrofolate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Sus scrofa tetrahydropteroylmonoglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
 Sus scrofa tetrahydropteroyltriglutamate + L-methionine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.13 37
-
 2 h, complete loss of activity Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.13 5
-
 unstable Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.13 S-adenosyl-L-methionine required Sus scrofa
2.1.1.13 vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Sus scrofa