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Literature summary extracted from

  • Hatch, M.D.; Turner, J.F.
    A protein disulfide reductase from pea seeds (1960), Biochem. J., 76, 556-562.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.8 AgNO3 0.009 mM, 66% inhibition Lathyrus oleraceus
1.8.1.8 arsenite 0.15 mM, complete inhibition Lathyrus oleraceus
1.8.1.8 CdSO4 0.005 mM, 91% inhibition Lathyrus oleraceus
1.8.1.8 CuSO4 0.015 mM, complete inhibition Lathyrus oleraceus
1.8.1.8 HgCl2 0.002 mM, 92% inhibition Lathyrus oleraceus
1.8.1.8 iodoacetate 0.054 mM, 57% inhibition Lathyrus oleraceus
1.8.1.8 p-chloromercuribenzoate 0.054 mM, 88% inhibition Lathyrus oleraceus
1.8.1.8 ZnSO4 0.003 mM, 82% inhibition Lathyrus oleraceus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.8 0.002
-
 NADPH
-
 Lathyrus oleraceus

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.8 Avena sativa
-
-
-
1.8.1.8 Hordeum vulgare
-
-
-
1.8.1.8 Lathyrus oleraceus
-
-
-
1.8.1.8 Triticum aestivum
-
-
-
1.8.1.8 Vicia faba
-
-
-
1.8.1.8 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.8
-
 Lathyrus oleraceus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.8 root
-
 Lathyrus oleraceus
-
1.8.1.8 root
-
 Hordeum vulgare
-
1.8.1.8 root
-
 Zea mays
-
1.8.1.8 seed
-
 Lathyrus oleraceus
-
1.8.1.8 seed
-
 Avena sativa
-
1.8.1.8 seed
-
 Vicia faba
-
1.8.1.8 seed
-
 Triticum aestivum
-
1.8.1.8 seed
-
 Hordeum vulgare
-
1.8.1.8 seed
-
 Zea mays
-
1.8.1.8 shoot
-
 Lathyrus oleraceus
-
1.8.1.8 shoot
-
 Vicia faba
-
1.8.1.8 shoot
-
 Triticum aestivum
-
1.8.1.8 shoot
-
 Hordeum vulgare
-
1.8.1.8 shoot
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.8 additional information
-
-
 Lathyrus oleraceus

Storage Stability

EC Number Storage Stability Organism
1.8.1.8 -15°C, 8 weeks, stable Lathyrus oleraceus
1.8.1.8 -15°C, 8 weeks, stable Avena sativa
1.8.1.8 -15°C, 8 weeks, stable Vicia faba
1.8.1.8 -15°C, 8 weeks, stable Triticum aestivum
1.8.1.8 -15°C, 8 weeks, stable Hordeum vulgare
1.8.1.8 -15°C, 8 weeks, stable Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.8 NAD(P)H + protein disulfide
-
 Vicia faba NAD(P)+ + protein dithiol
-
 ?
1.8.1.8 NAD(P)H + protein disulfide
-
 Triticum aestivum NAD(P)+ + protein dithiol
-
 ?
1.8.1.8 NAD(P)H + protein disulfide
-
 Hordeum vulgare NAD(P)+ + protein dithiol
-
 ?
1.8.1.8 NAD(P)H + protein disulfide
-
 Zea mays NAD(P)+ + protein dithiol
-
 ?
1.8.1.8 NAD(P)H + protein disulfide
-
 Avena sativa NAD(P)+ + protein dithiol
-
 ?
1.8.1.8 NAD(P)H + protein disulfide reaction rate with NADPH is approximately twice that with NADH Lathyrus oleraceus NAD(P)+ + protein dithiol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.1.8 60
-
 10 min, stable Lathyrus oleraceus
1.8.1.8 70
-
 10 min, complete inactivation Lathyrus oleraceus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.8 6.9 7.4
-
 Lathyrus oleraceus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.1.8 6.2 7.8 pH 6.2: about 50% of activity maximum, pH 7.8: about 20% of activity maximum Lathyrus oleraceus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.8.1.8 5.5
-
 5°C or 22°C, irreversible inactivation Lathyrus oleraceus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.8 NADH reaction rate with NADPH is approximately twice of that with NADH Lathyrus oleraceus
1.8.1.8 NADPH reaction rate with NADPH is approximately twice of that with NADH Lathyrus oleraceus