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Literature summary extracted from

  • Baas, D.; Retey, J.
    Cloning, sequencing and heterologous expression of pyrogallol-phloroglucinol transhydroxylase from Pelobacter acidigallici (1999), Eur. J. Biochem., 265, 896-901.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.97.1.2 expression in Escherichia coli strain BL21 and M15 Pelobacter acidigallici

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.97.1.2 Fe iron-sulfur cluster Pelobacter acidigallici

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.97.1.2 31221
-
alpha,beta, 1 * 99260 + 1 * 31221, calculation from amino acid sequence Pelobacter acidigallici
1.97.1.2 99260
-
alpha,beta, 1 * 99260 + 1 * 31221, calculation from amino acid sequence Pelobacter acidigallici
1.97.1.2 130500
-
excluding cofactors, calculation from amino acid sequence Pelobacter acidigallici

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.97.1.2 1,2,3,5-tetrahydroxybenzene + pyrogallol Pelobacter acidigallici key step in the anaerobic degradation of gallic acid and releated polyphenolic compounds ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.97.1.2 Pelobacter acidigallici
-
ATCC 49970
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.97.1.2 1,2,3,5-tetrahydroxybenzene + pyrogallol pyrogallol i.e. 1,2,3-tri-hydroxybenzene Pelobacter acidigallici phloroglucinol + 1,2,3,5-tetrahydroxybenzene phloroglucinol i.e. 1,3,5-tri-hydroxybenzene ?
1.97.1.2 1,2,3,5-tetrahydroxybenzene + pyrogallol key step in the anaerobic degradation of gallic acid and releated polyphenolic compounds Pelobacter acidigallici ?
-
?
1.97.1.2 additional information water is not the source of the added hydroxyl groups Pelobacter acidigallici ?
-
?

Subunits

EC Number Subunits Comment Organism
1.97.1.2 heterodimer alpha,beta, 1 * 99260 + 1 * 31221, calculation from amino acid sequence Pelobacter acidigallici

Cofactor

EC Number Cofactor Comment Organism Structure
1.97.1.2 molybdenum cofactor
-
Pelobacter acidigallici