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Literature summary extracted from

  • Yang, Y.S.; Broadwater, J.A.; Pulver, S.C.; Fox, B.G.; Solomon, E.I.
    Circular dichroism and magnetic circular dichroism studies of the reduced binuclear non-heme iron site of stearoyl-ACP DELTA9-desaturase: substrate binding and comparison to ribonucleotide reductase (1999), J. Am. Chem. Soc., 121, 2770-2783.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.1 coexpression of acyl carrier protein isoform I gene and holo-acyl carrier protein synthase of Escherichia coli Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.1 membrane bound Spinacia oleracea 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.19.1 Fe2+ each subunit of homodimer contains one binuclear non-heme iron active site. The reduced DELTA9-desaturase has two approximately equivalent 5-coordinate irons in a distorted square pyramidal geometry, a two-in-two-out equatorial distortion, the irons are rhombic, weakly antiferromagnetically coupled. Addition of substrate causes dramatic changes, first ion remains 5-coordinate but is distorted toward a trigonal bipyramidal structure, second iron changes to 4-coordinate Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.1 40000
-
 homodimer, 2 * 40000 Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.1 stearoyl-acyl-carrier protein + AH2 + O2 Spinacia oleracea insertion of a cis double bond between the 9 and 10 position, essential step in fatty acid biosynthesis oleoyl-acyl-carrier protein + A + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.1 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.1
-
 Spinacia oleracea

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.19.1 stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O the possible mechanism would be that dioxygen has to bind to both irons for effective two-electron transfer to the half-occupied antibonding pi orbitals of dioxygen to give a peroxide level intermediate Spinacia oleracea
a

Storage Stability

EC Number Storage Stability Organism
1.14.19.1 reduced enzyme is stable under O2 for hours Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.1 stearoyl-acyl-carrier protein + AH2 + O2 insertion of a cis double bond between the 9 and 10 position, essential step in fatty acid biosynthesis Spinacia oleracea oleoyl-acyl-carrier protein + A + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.1 dimer homodimer, 2 * 40000 Spinacia oleracea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.19.1 0.5
-
 stearoyl-acyl-carrier protein
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.1 NADPH
-
 Spinacia oleracea