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Literature summary extracted from

  • Harlocker, S.L.; Kapper, M.A.; Greenwalt, D.E.; Bishop, S.H.
    Phosphoenolpyruvate carboxykinase from ribbed mussel gill tissue: reactivity with metal ions, kinetics, and action of 3-mercaptopicolinic acid (1991), J. Exp. Zool., 257, 285-298.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.32 3-Aminopicolinic acid
-
 Modiolus demissus
4.1.1.32 3-Mercaptopicolinic acid strong Modiolus demissus
4.1.1.32 ADP uncompetitive with respect to IDP, partially competitive with respect to phosphoenolpyruvate Modiolus demissus
4.1.1.32 ATP strong Modiolus demissus
4.1.1.32 Hydroxymalonate slight Modiolus demissus
4.1.1.32 KCl 120 mM, 50% inhibition Modiolus demissus
4.1.1.32 NaCl 115 mM, 50% inhibition Modiolus demissus
4.1.1.32 Zn2+ activates at low levels, inhibits above 0.3 mM Modiolus demissus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.32 0.024
-
 GDP in presence of 1 mM MnCl2 and 1 mM MgCl2 Modiolus demissus
4.1.1.32 0.024
-
 oxaloacetate in presence of 1 mM MnCl2 and 1 mM MgCl2 Modiolus demissus
4.1.1.32 0.046
-
 IDP in presence of 1 mM MnCl2 and 1 mM MgCl2 Modiolus demissus
4.1.1.32 0.055
-
 GTP in presence of 1 mM MnCl2 and 1 mM MgCl2 Modiolus demissus
4.1.1.32 0.396
-
 phosphoenolpyruvate in presence of 1 mM MnCl2 and 1 mM MgCl2 Modiolus demissus
4.1.1.32 13
-
 CO2 in presence of 1 mM MnCl2 and 1 mM MgCl2 Modiolus demissus
4.1.1.32 13
-
 CO2 CO2 in form of HCO3- Modiolus demissus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.32 cytosol
-
 Modiolus demissus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.32 Co2+ Mn2+ or Co2+ required Modiolus demissus
4.1.1.32 Mn2+ Km: 0.28 mM Modiolus demissus
4.1.1.32 Mn2+ Mn2+ or Co2+ required Modiolus demissus
4.1.1.32 Zn2+ activates at low levels, inhibits above 0.3 mM Modiolus demissus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.32 70000
-
 gel filtration Modiolus demissus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.32 Modiolus demissus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.32 1.81
-
-
 Modiolus demissus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.32 GDP + phosphoenolpyruvate + CO2
-
 Modiolus demissus GTP + oxaloacetate
-
 ?
4.1.1.32 GTP + oxaloacetate
-
 Modiolus demissus GDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.32 IDP + phosphoenolpyruvate + CO2
-
 Modiolus demissus ITP + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.32 monomer 1 * 70000, SDS-PAGE Modiolus demissus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.32 6.6
-
-
 Modiolus demissus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.32 6.3 7.5 about 50% of maximal activity at pH 6.3 and pH 7.5 Modiolus demissus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.32 115
-
 NaCl
-
 Modiolus demissus
4.1.1.32 120
-
 KCl
-
 Modiolus demissus