Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Imbault, P.; Sarantoglou, V.; Weil, J.H.
    Properties of purified chloroplastic and cytoplasmic valyl- and leucyl-tRNA synthetases from Euglena gracilis (1982), Phytochemistry, 21, 1189-1193.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.4 spermidine in absence of Mg2+ spermine allows a very low level of cytoplasmic enzyme Euglena gracilis
6.1.1.9 spermine chloroplastic enzyme: unable to promote aminoacylation in absence of Mg2+. In presence of suboptimal concentrations of Mg2+, spermine can restore maximal activity (not in cytoplasmic enzyme) Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.9 KCl above 30 mM: inhibits activity of cytoplasmic enzyme more strongly than that of chloroplastic enzyme, 30 mM: enhances activity of the cytoplasmic enzyme, has no effect on the chloroplastic enzyme Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.9 0.00005
-
 tRNAVal of Euglena gracilis Euglena gracilis
6.1.1.9 0.00005
-
 tRNAVal cytoplasmic enzyme Euglena gracilis
6.1.1.9 0.00006
-
 tRNAVal of Euglena gracilis, , chloroplastic enzyme Euglena gracilis
6.1.1.9 0.00008
-
 tRNAVal tRNA, of E. coli, , chloroplasmic enzyme Euglena gracilis
6.1.1.9 0.00008
-
 tRNAVal tRNA, of yeast, , cytoplasmic enzyme Euglena gracilis
6.1.1.9 0.015
-
 L-valine chloroplastic enzyme Euglena gracilis
6.1.1.9 0.05
-
 L-valine cytoplasmic enzyme Euglena gracilis
6.1.1.9 0.05
-
 L-valine ATP, cytoplasmic enzyme Euglena gracilis
6.1.1.9 0.07
-
 ATP cytoplasmic enzyme Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.4 chloroplast
-
 Euglena gracilis 9507
-
6.1.1.4 cytoplasm
-
 Euglena gracilis 5737
-
6.1.1.9 cytoplasm
-
 Euglena gracilis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.4 Ca2+ can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme) Euglena gracilis
6.1.1.4 Co2+ can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme Euglena gracilis
6.1.1.4 K+ 30 mM KCl enhance activity of cytoplasmic enzyme, no effect on chloroplastic enzyme, concentrations higher than 30 mM inhibit cytoplasmic enzyme more strongly than chloroplastic enzyme Euglena gracilis
6.1.1.4 Mn2+ can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme) Euglena gracilis
6.1.1.9 Ca2+ no activity Euglena gracilis
6.1.1.9 Co2+ can partially replace Mg2+ in reaction with chloroplastic enzyme only Euglena gracilis
6.1.1.9 KCl 30 mM: enhances activity of the cytoplasmic enzyme, has no effect on the chloroplastic enzyme, above 30 mM: inhibits activity of cytoplasmic enzyme more strongly than that of chloroplastic enzyme Euglena gracilis
6.1.1.9 Mg2+ divalent cation required, Mg2+ is most effective Euglena gracilis
6.1.1.9 Mg2+ optimal Mg2+/ATP ratio is 2.5 for the chloroplastic and 1 for the cytoplasmic enzyme Euglena gracilis
6.1.1.9 Mn2+ 60% of the activation with Mg2+ Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Euglena gracilis
-
-
-
6.1.1.9 Euglena gracilis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu chloroplastic enzyme: high specificity towards tRNAs, in contrast the cytoplasmic enzyme recognizes tRNAs from the bleached mutant and from yeast, but also some tRNALeu isoacceptors from E. coli Euglena gracilis AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.9 ATP + L-valine + tRNAVal chloroplastic enzyme charges E. coli tRNA and not yeast tRNA, cytoplasmic enzyme is able to aminoacylate yeast tRNA but not E. coli tRNA Euglena gracilis AMP + diphosphate + L-valyl-tRNAVal
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.9 7.5 8 Tris-HCl or cacodylate buffer Euglena gracilis