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Literature summary extracted from

  • Miyatake, K.; Ito, T.; Kitaoka, S.
    Subcellular location and some properties of phosphoenolpyruvate carboxykinase (PEPCK) in Euglena gracilis (1984), Agric. Biol. Chem., 48, 2139-2141.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.32 0.014
-
 oxaloacetate
-
 Euglena gracilis
4.1.1.32 0.05
-
 ITP
-
 Euglena gracilis
4.1.1.32 0.059
-
 GDP
-
 Euglena gracilis
4.1.1.32 0.065
-
 GTP
-
 Euglena gracilis
4.1.1.32 0.066
-
 CO2 CO2 in form of HCO3- Euglena gracilis
4.1.1.32 0.13
-
 phosphoenolpyruvate
-
 Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.32 cytosol
-
 Euglena gracilis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.32 Co2+ activates Euglena gracilis
4.1.1.32 Co2+ Km: 0.055 mM Euglena gracilis
4.1.1.32 Mg2+ activates Euglena gracilis
4.1.1.32 Mg2+ maximal activity at 1 mM Euglena gracilis
4.1.1.32 Mg2+ Km: 0.043 mM Euglena gracilis
4.1.1.32 Mn2+ activates Euglena gracilis
4.1.1.32 Mn2+ maximal activity at 1 mM Euglena gracilis
4.1.1.32 Mn2+ Km: 0.045 mM Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.32 Euglena gracilis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.32 GDP + phosphoenolpyruvate + CO2
-
 Euglena gracilis GTP + oxaloacetate
-
 ?
4.1.1.32 GTP + oxaloacetate
-
 Euglena gracilis GDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.32 IDP + phosphoenolpyruvate + CO2
-
 Euglena gracilis ITP + oxaloacetate
-
 r
4.1.1.32 ITP + oxaloacetate
-
 Euglena gracilis IDP + phosphoenolpyruvate + CO2
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.32 35 40
-
 Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.32 6.5
-
 carboxylation Euglena gracilis
4.1.1.32 8.5
-
 decarboxylation Euglena gracilis