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Literature summary extracted from

  • Souciet, G.; Dietrich, A.; Colas, B.; Razafimahatratra, P.; Weil, J.H.
    Purification and properties of chloroplast leucyl-tRNA synthetase from a higher plant. Phaseolus vulgaris (1982), J. Biol. Chem., 257, 9598-9604.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.00085
-
 tRNALeu substrate from E. coli Phaseolus vulgaris
6.1.1.4 0.0026
-
 tRNALeu unfractionated substrate of E. coli Phaseolus vulgaris
6.1.1.4 0.014
-
 L-Leu
-
 Phaseolus vulgaris
6.1.1.4 0.18
-
 ATP
-
 Phaseolus vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.4 chloroplast
-
 Phaseolus vulgaris 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.4 K+ slight stimulation by 50 mM KCl, progressive inhibition with higher concentrations Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 122000
-
 1 * 122000, urea-SDS-PAGE Phaseolus vulgaris
6.1.1.4 135000
-
 gel filtration Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Phaseolus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4
-
 Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.4 leaf
-
 Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.4 1.55
-
-
 Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Phaseolus vulgaris AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 monomer 1 * 122000, urea-SDS-PAGE Phaseolus vulgaris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 3.15
-
 Leu-tRNALeu
-
 Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.4 9
-
-
 Phaseolus vulgaris