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Literature summary extracted from

  • Goto, Y.; Shimizu, J.; Okazaki, T.; Shukuya, R.
    Purification and characterization of cytosol phosphoenolpyruvate carboxykinase from bullfrog (Rana catesbeiana) liver (1979), J. Biochem., 86, 71-78.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.32 0.004
-
 oxaloacetate pH 7.2 Lithobates catesbeianus
4.1.1.32 0.067
-
 GTP exchange reaction Lithobates catesbeianus
4.1.1.32 0.2
-
 ITP exchange reaction Lithobates catesbeianus
4.1.1.32 0.8
-
 phosphoenolpyruvate pH 7.2 Lithobates catesbeianus
4.1.1.32 0.8
-
 IDP pH 6.3 Lithobates catesbeianus
4.1.1.32 0.8
-
 GDP at pH 6.3 or at pH 7.2 Lithobates catesbeianus
4.1.1.32 1
-
 oxaloacetate exchange reaction Lithobates catesbeianus
4.1.1.32 1.6
-
 GTP pH 7.2 Lithobates catesbeianus
4.1.1.32 2.5
-
 IDP pH 7.2 Lithobates catesbeianus
4.1.1.32 4
-
 ITP pH 7.2 Lithobates catesbeianus
4.1.1.32 8
-
 CO2 pH 6.3 Lithobates catesbeianus
4.1.1.32 8
-
 CO2 CO2 in form of HCO3- Lithobates catesbeianus
4.1.1.32 9.6
-
 phosphoenolpyruvate pH 6.3 Lithobates catesbeianus
4.1.1.32 31
-
 CO2 exchange reaction Lithobates catesbeianus
4.1.1.32 31
-
 CO2 CO2 in form of HCO3- Lithobates catesbeianus
4.1.1.32 46
-
 CO2 pH 7.2 Lithobates catesbeianus
4.1.1.32 46
-
 CO2 CO2 in form of HCO3- Lithobates catesbeianus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.32 cytosol
-
 Lithobates catesbeianus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.32 Mg2+ can replace Mn2+ in activation of oxaloacetate decarboxylation Lithobates catesbeianus
4.1.1.32 Mn2+ absolutely required for phosphoenolpyruvate carboxylation Lithobates catesbeianus
4.1.1.32 Mn2+ half-maximal activation: of phosphoenolpyruvate carboxylation at 2 mM, of oxaloacetate decarboxylation at 0.4 mM, of exchange reaction at 8 mM Lithobates catesbeianus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.32 72000
-
 glycerol gradient centrifugation Lithobates catesbeianus
4.1.1.32 75000
-
 1 * 75000, SDS-PAGE Lithobates catesbeianus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.32 Lithobates catesbeianus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.32
-
 Lithobates catesbeianus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.32 liver
-
 Lithobates catesbeianus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.32 GDP + phosphoenolpyruvate + CO2
-
 Lithobates catesbeianus GTP + oxaloacetate
-
 r
4.1.1.32 GTP + oxaloacetate
-
 Lithobates catesbeianus GDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.32 IDP + phosphoenolpyruvate + CO2
-
 Lithobates catesbeianus ITP + oxaloacetate
-
 ?
4.1.1.32 ITP + oxaloacetate
-
 Lithobates catesbeianus IDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.32 additional information catalyzes an exchange reaction between HCO3- and the beta-carboxyl group of oxaloacetate Lithobates catesbeianus ?
-
 ?
4.1.1.32 additional information ADP is completely inactive in carboxylation of oxaloacetate Lithobates catesbeianus ?
-
 ?
4.1.1.32 UDP + phosphoenolpyruvate + CO2 very low activity Lithobates catesbeianus UTP + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.32 monomer 1 * 75000, SDS-PAGE Lithobates catesbeianus