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Literature summary extracted from

  • Sadaie, M.; Nagano, T.; Suzuki, T.; Shinoyama, H.; Fujii, T.
    Some properties and physiological roles of phosphoenolpyruvate carboxylase in Rhodopseudomonas sp. No. 7 grown on ethanol under anaerobic-light conditions (1997), Biosci. Biotechnol. Biochem., 61, 625-630.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.31 dioxane
-
 Rhodopseudomonas sp.
4.1.1.31 ethanol activates Rhodopseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 ADP
-
 Rhodopseudomonas sp.
4.1.1.31 Asp
-
 Rhodopseudomonas sp.
4.1.1.31 ATP
-
 Rhodopseudomonas sp.
4.1.1.31 GTP
-
 Rhodopseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.31 0.23
-
 phosphoenolpyruvate in presence of 0.04 mM acetyl-CoA Rhodopseudomonas sp.
4.1.1.31 1.7
-
 HCO3- in presence of 0.04 mM acetyl-CoA Rhodopseudomonas sp.
4.1.1.31 3.7
-
 HCO3-
-
 Rhodopseudomonas sp.
4.1.1.31 5
-
 phosphoenolpyruvate
-
 Rhodopseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.31 Mg2+ Mg2+ or Mn2+ required Rhodopseudomonas sp.
4.1.1.31 Mn2+ Mg2+ or Mn2+ required Rhodopseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 102000
-
 4 * 102000, SDS-PAGE Rhodopseudomonas sp.
4.1.1.31 400000
-
 gel filtration Rhodopseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Rhodopseudomonas sp.
-
-
-
4.1.1.31 Rhodopseudomonas sp. No.7
-
-
-
4.1.1.49 Rhodopseudomonas sp.
-
 No. 7
-
4.1.1.49 Rhodopseudomonas sp. No. 7
-
 No. 7
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
 Rhodopseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.31 culture condition:acetate-grown cell grown photoanaerobically Rhodopseudomonas sp.
-
4.1.1.31 culture condition:ethanol-grown cell grown photoanaerobically Rhodopseudomonas sp.
-
4.1.1.49 culture condition:acetate-grown cell grown photoanaerobically Rhodopseudomonas sp.
-
4.1.1.49 culture condition:ethanol-grown cell grown photoanaerobically Rhodopseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 48.6
-
-
 Rhodopseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
4.1.1.31 -20°C, little loss of activity after 8 months Rhodopseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
 Rhodopseudomonas sp. phosphate + oxaloacetate
-
 ?
4.1.1.31 phosphoenolpyruvate + CO2
-
 Rhodopseudomonas sp. No. 7 phosphate + oxaloacetate
-
 ?
4.1.1.31 phosphoenolpyruvate + CO2
-
 Rhodopseudomonas sp. No.7 phosphate + oxaloacetate
-
 ?
4.1.1.31 phosphoenolpyruvate + HCO3-
-
 Rhodopseudomonas sp. phosphate + oxaloacetate
-
 ?
4.1.1.31 phosphoenolpyruvate + HCO3-
-
 Rhodopseudomonas sp. No. 7 phosphate + oxaloacetate
-
 ?
4.1.1.31 phosphoenolpyruvate + HCO3-
-
 Rhodopseudomonas sp. No.7 phosphate + oxaloacetate
-
 ?
4.1.1.49 ADP + phosphoenolpyruvate + CO2
-
 Rhodopseudomonas sp. ATP + oxaloacetate
-
 ?
4.1.1.49 ADP + phosphoenolpyruvate + CO2
-
 Rhodopseudomonas sp. No. 7 ATP + oxaloacetate
-
 ?
4.1.1.49 ADP + phosphoenolpyruvate + CO2
-
 Rhodopseudomonas sp. No.7 ATP + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 tetramer 4 * 102000, SDS-PAGE Rhodopseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.31 50
-
-
 Rhodopseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.31 45
-
 rapid decrease of activity above Rhodopseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.31 9
-
-
 Rhodopseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.31 acetyl-CoA
-
 Rhodopseudomonas sp.