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Literature summary extracted from

  • Colas, B.; Imbault, P.; Sarantoglou, V.; Boulanger, Y.; Weil, J.H.
    Chloroplastic and cytoplasmic valyl- and leucyl-tRNA synthetases from Euglena gracilis. Comparative study of their structural properties (1982), Biochim. Biophys. Acta, 697, 71-77.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.1.1.4 20-30% loss of activity after 1 cycle of freezing and thawing Euglena gracilis
6.1.1.4 stability is lower with decreasing protein concentration Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.4 chloroplast
-
 Euglena gracilis 9507
-
6.1.1.4 cytoplasm
-
 Euglena gracilis 5737
-
6.1.1.9 cytoplasm
-
 Euglena gracilis 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 100000
-
 1 * 100000, chloroplastic enzyme, SDS-PAGE Euglena gracilis
6.1.1.4 116000
-
 1 * 116000, cytoplasmic enzyme, SDS-PAGE Euglena gracilis
6.1.1.9 126000
-
 1 * 126000, urea-SDS-PAGE Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Euglena gracilis
-
-
-
6.1.1.9 Euglena gracilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4
-
 Euglena gracilis
6.1.1.9
-
 Euglena gracilis

Storage Stability

EC Number Storage Stability Organism
6.1.1.4 4°C, 50 mM potassium phosphate buffer, pH 7.0, 1 mM MgCl2, 0.002 mM L-leucine, 100 mM NaCl, 15% v/v 1,2-propanediol, stable for several weeks Euglena gracilis
6.1.1.9 4°C, 50 mM potassium phosphate buffer, pH 7.0, 1 mM MgCl2, 0.002 mM L-valine, 100 mM NaCl, 15% v/v 1,2-propanediol, stable for several weeks Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Euglena gracilis AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.9 ATP + L-valine + tRNAVal
-
 Euglena gracilis AMP + diphosphate + L-valyl-tRNAVal
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 monomer 1 * 116000, cytoplasmic enzyme, SDS-PAGE Euglena gracilis
6.1.1.4 monomer 1 * 100000, chloroplastic enzyme, SDS-PAGE Euglena gracilis
6.1.1.9 monomer 1 * 126000, urea-SDS-PAGE Euglena gracilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.9 40
-
 5 min, chloroplastic enzyme loses 30% of its activity, mitochondrial enzyme 75% Euglena gracilis