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Literature summary extracted from

  • Nakamura, T.; Minoguchi, S.; Izui, K.
    Purification and characterization of recombinant phosphoenolpyruvate carboxylase of Thermus sp. (1996), J. Biochem., 120, 518-524.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.31 expression in Escherichia coli Thermus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 ADP
-
 Thermus sp.
4.1.1.31 Asp
-
 Thermus sp.
4.1.1.31 ATP
-
 Thermus sp.
4.1.1.31 fructose 1,6-diphosphate
-
 Thermus sp.
4.1.1.31 glucose 6-phosphate
-
 Thermus sp.
4.1.1.31 GTP
-
 Thermus sp.
4.1.1.31 guanidine hydrochloride
-
 Thermus sp.
4.1.1.31 malate
-
 Thermus sp.
4.1.1.31 phosphate
-
 Thermus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 100000
-
 4 * 100000, SDS-PAGE Thermus sp.
4.1.1.31 400000
-
 gel filtration Thermus sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Thermus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
 Thermus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 29.4
-
-
 Thermus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
 Thermus sp. phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 tetramer 4 * 100000, SDS-PAGE Thermus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.31 70
-
 in presence of 0.3 M acetyl-CoA and 2 mM phosphoenolpyruvate Thermus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.31 50 80 about 50% of maximal activity at 50°C and 80°C Thermus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.31 85
-
 half-life: 85 min Thermus sp.
4.1.1.31 90
-
 half-life: 110 min Thermus sp.
4.1.1.31 95
-
 half-life: 50 min Thermus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.31 8.6
-
 at 30°C Thermus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.31 additional information
-
-
 Thermus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.31 acetyl-CoA strongly dependent on the allosteric activator Thermus sp.