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Literature summary extracted from

  • Imbault, P.; Colas, B.; Sarantoglou, V.; Boulanger, Y.; Weil, J.H.
    Chloroplast leucyl-tRNA synthetase from Euglena gracilis. Purification, kinetic analysis, and structural characterization (1981), Biochemistry, 20, 5855-5859.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.0013
-
 tRNALeu
-
 Euglena gracilis
6.1.1.4 0.008
-
 L-Leu
-
 Euglena gracilis
6.1.1.4 0.13
-
 ATP
-
 Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.4 chloroplast
-
 Euglena gracilis 9507
-
6.1.1.4 cytoplasm
-
 Euglena gracilis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.4 Ca2+ can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme) Euglena gracilis
6.1.1.4 Ca2+ 75% of the maximal activity obtained with Mg2+ Euglena gracilis
6.1.1.4 Co2+ can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme Euglena gracilis
6.1.1.4 Co2+ 40% of the maximal activity obtained with Mg2+ Euglena gracilis
6.1.1.4 Mg2+ activates Euglena gracilis
6.1.1.4 Mg2+ optimal Mg2+/ATP ratio: 1.2 Euglena gracilis
6.1.1.4 Mn2+ can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme) Euglena gracilis
6.1.1.4 Mn2+ 75% of the maximal activity obtained with Mg2+ Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 100000
-
 gel filtration Euglena gracilis
6.1.1.4 116000
-
 gel filtration Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Euglena gracilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4
-
 Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.4 1.233
-
-
 Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Euglena gracilis AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 monomer 1 * 100000, urea-SDS-PAGE Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.4 8
-
-
 Euglena gracilis