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Literature summary extracted from

  • Nimmo, G.A.; Nimmo, H.G.; Hamilton, I.D.; Fewson, C.A.; Wilkins, M.B.
    Purification of the phosphorylated night form and dephosphorylated day form of phosphoenolpyruvate carboxylase from Bryophyllum fedtschenkoi (1986), Biochem. J., 239, 213-220.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 malate the day form of the enzyme is 10times more sensitive than the night form of the enzyme Kalanchoe fedtschenkoi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.31 phosphate the night form of the enzyme is phosphorylated, the phosphate is covalently bound to Ser, the day form of the enzyme is dephosphorylated Kalanchoe fedtschenkoi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 112000
-
 4 * 112000, SDS-PAGE Kalanchoe fedtschenkoi
4.1.1.31 310000
-
 gel filtration in presence of malate Kalanchoe fedtschenkoi
4.1.1.31 370000
-
 gel filtration in absence of malate Kalanchoe fedtschenkoi

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Kalanchoe fedtschenkoi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31 2 forms: a night form and a day form Kalanchoe fedtschenkoi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 21
-
-
 Kalanchoe fedtschenkoi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
 Kalanchoe fedtschenkoi phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 tetramer 4 * 112000, SDS-PAGE Kalanchoe fedtschenkoi