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Literature summary extracted from
- Alfalfa root nodule carbon dioxide fixation. II. Partial purification and characterization of root nodule phosphoenolpyruvate carboxylase (1984), Plant Physiol., 75, 261-264.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.31 | AMP | slight | Medicago sativa |  |
| 4.1.1.31 | Ca2+ | substitution of 20 mM CaCl2 for 20 mM MgCl2 results in 90-100% inhibition | Medicago sativa | |
| 4.1.1.31 | malate | - |
Medicago sativa | |
| 4.1.1.31 | Mn2+ | substitution of 20 mM MnCl2 for 20 mM MgCl2 results in 90-100% inhibition | Medicago sativa | |
| 4.1.1.31 | Zn2+ | substitution of 20 mM ZnCl2 for 20 mM MgCl2 results in 90-100% inhibition | Medicago sativa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.31 | 0.21 | - |
phosphoenolpyruvate | - |
Medicago sativa | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.1.1.31 | cytosol | - |
Medicago sativa | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.31 | Mg2+ | Km: 0.1 mM | Medicago sativa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.31 | 97000 | - |
x * 97000, SDS-PAGE | Medicago sativa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.31 | Medicago sativa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.1.31 | partial | Medicago sativa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.1.1.31 | root nodule | - |
Medicago sativa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.31 | 42.3 | - |
- |
Medicago sativa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.31 | phosphoenolpyruvate + CO2 | - |
Medicago sativa | phosphate + oxaloacetate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.1.31 | ? | x * 97000, SDS-PAGE | Medicago sativa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.31 | 7.5 | - |
- |
Medicago sativa |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.31 | 7 | 9 | 50% of maximal activity at pH 7.0 and at pH 9.0 | Medicago sativa |
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Release 2023.1 (January 2023)
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