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Literature summary extracted from

  • Jones, R.; Wilkins, M.B.; Coggins, J.R.; Fewson, C.A.; Malcolm, A.D.B.
    Phosphoenolpyruvate carboxylase from the crassulacean plant Bryophyllum fedtschenkoi Hamet et Perrier (1978), Biochem. J., 175, 391-406.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.31 Polyethylene glycol required for maximal activity Kalanchoe fedtschenkoi

General Stability

EC Number General Stability Organism
4.1.1.31 thiol protecting agents stabilize during purification Kalanchoe fedtschenkoi

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 malate
-
Kalanchoe fedtschenkoi
4.1.1.31 phosphate
-
Kalanchoe fedtschenkoi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.31 0.7
-
phosphoenolpyruvate in absence of effectors, at 25°C Kalanchoe fedtschenkoi
4.1.1.31 1.7
-
phosphoenolpyruvate at 14°C Kalanchoe fedtschenkoi
4.1.1.31 2.4
-
phosphoenolpyruvate at 40°C Kalanchoe fedtschenkoi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 105000
-
4 * 105000, SDS-PAGE Kalanchoe fedtschenkoi
4.1.1.31 500000
-
gel filtration Kalanchoe fedtschenkoi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.31 Phosphoenolpyruvate + CO2 Kalanchoe fedtschenkoi possible role of the enzyme in controlling a circadian rhythm of CO2 fixation ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Kalanchoe fedtschenkoi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
Kalanchoe fedtschenkoi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 26
-
-
Kalanchoe fedtschenkoi

Storage Stability

EC Number Storage Stability Organism
4.1.1.31 4°C, in (NH4)2SO4 suspension, 1 month stable Kalanchoe fedtschenkoi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
Kalanchoe fedtschenkoi phosphate + oxaloacetate
-
?
4.1.1.31 Phosphoenolpyruvate + CO2 possible role of the enzyme in controlling a circadian rhythm of CO2 fixation Kalanchoe fedtschenkoi ?
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 tetramer 4 * 105000, SDS-PAGE Kalanchoe fedtschenkoi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.31 50
-
10 min, only small activity loss below 50°C Kalanchoe fedtschenkoi
4.1.1.31 55 57 10 min, 50% denaturation Kalanchoe fedtschenkoi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.31 5.8
-
double optimum at pH 5.8 and pH 7.8 Kalanchoe fedtschenkoi
4.1.1.31 7.8
-
double optimum at pH 5.8 and at pH 7.8 Kalanchoe fedtschenkoi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.31 5.8 7.8 double optimum at pH 5.8 and at pH 7.8, minimum at pH 6.6 Kalanchoe fedtschenkoi