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Literature summary extracted from

  • Miziorko, H.M.; Nowak, T.; Mildvan, A.S.
    Spinach leaf phosphoenolpyruvate carboxylase: purification, properties, and kinetic studies (1974), Arch. Biochem. Biophys., 163, 378-389.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.1.31 desalted enzyme, in Tris buffer, pH 7.5, room temperature, half-life is 3-4 h Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 D-Phospholactate
-
Spinacia oleracea
4.1.1.31 L-lactate
-
Spinacia oleracea
4.1.1.31 L-phospholactate
-
Spinacia oleracea
4.1.1.31 methyl phosphate
-
Spinacia oleracea
4.1.1.31 oxalate
-
Spinacia oleracea
4.1.1.31 phosphite
-
Spinacia oleracea
4.1.1.31 Phosphoglycolate
-
Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.31 0.1
-
phosphoenolpyruvate with Mn2+ as activator Spinacia oleracea
4.1.1.31 0.108
-
phosphoenolpyruvate with Mg2+ as activator Spinacia oleracea
4.1.1.31 1.1
-
phosphoenolpyruvate without activator Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.31 Mg2+ activates Spinacia oleracea
4.1.1.31 Mn2+ activates Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 130000
-
4 * 130000, SDS-PAGE Spinacia oleracea
4.1.1.31 560000
-
gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 additional information
-
-
Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
4.1.1.31 4°C, in 50% ammonium sulfate, stable for 2-3 weeks Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
Spinacia oleracea phosphate + oxaloacetate
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 tetramer 4 * 130000, SDS-PAGE Spinacia oleracea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.31 43
-
phosphoenolpyruvate with Mn2+ as activator Spinacia oleracea
4.1.1.31 69
-
phosphoenolpyruvate with Mg2+ as activator Spinacia oleracea