Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Murasugi, A.; Hayashi, H.
    Purification and properties of leucyl-tRNA synthetase from Candida utilis (1975), Eur. J. Biochem., 57, 169-175.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.1.1.4 extremely unstable in absence of 2-mercaptoethanol Cyberlindnera jadinii

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.4 NEM
-
 Cyberlindnera jadinii
6.1.1.4 p-chloromercuribenzoate
-
 Cyberlindnera jadinii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.4 K+ optimal concentration: 80 mM KCl Cyberlindnera jadinii
6.1.1.4 Mg2+ activates Cyberlindnera jadinii
6.1.1.4 Mg2+ optimal concentration: 4 mM (with an ATP concentration of 2 mM) Cyberlindnera jadinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 128000
-
 1 * 128000, SDS-PAGE Cyberlindnera jadinii
6.1.1.4 145000
-
 disc gel electrophoresis Cyberlindnera jadinii
6.1.1.4 150000
-
 gel filtration Cyberlindnera jadinii

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Cyberlindnera jadinii
-
 Torulopsis
-
6.1.1.4 Cyberlindnera jadinii Torulopsis
-
 Torulopsis
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4
-
 Cyberlindnera jadinii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.4 3.92
-
-
 Cyberlindnera jadinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu apart from the homologous substrate the enzyme is able to aminoacylate pure E. coli tRNALeu Cyberlindnera jadinii AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeu apart from the homologous substrate the enzyme is able to aminoacylate pure E. coli tRNALeu Cyberlindnera jadinii Torulopsis AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 monomer 1 * 128000, SDS-PAGE Cyberlindnera jadinii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.4 30
-
 half-life: 4.5 min, in absence of 2-mercaptoethanol Cyberlindnera jadinii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 23.8
-
 Leu-tRNALeu
-
 Cyberlindnera jadinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.4 8
-
-
 Cyberlindnera jadinii