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Literature summary extracted from

  • Nakazawa, H.; Kumagai, H.; Yamada, H.
    Aromatic L-amino acid decarboxylase from Micrococcus percitreus. Purification, crystallization and properties (1981), Agric. Biol. Chem., 45, 2543-2552.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.28
-
 Micrococcus percitreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.28 0.98
-
 L-Phe
-
 Micrococcus percitreus
4.1.1.28 1.1
-
 L-Tyr
-
 Micrococcus percitreus
4.1.1.28 2.3
-
 3,4-dihydroxyphenylalanine
-
 Micrococcus percitreus
4.1.1.28 2.4
-
 L-Trp
-
 Micrococcus percitreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.28 48000
-
 2 * 48000, SDS-PAGE Micrococcus percitreus
4.1.1.28 101000
-
 gel filtration Micrococcus percitreus
4.1.1.28 105000
-
 equilibrium sedimentation Micrococcus percitreus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.28 Micrococcus percitreus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.28
-
 Micrococcus percitreus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.28 37.7
-
-
 Micrococcus percitreus

Storage Stability

EC Number Storage Stability Organism
4.1.1.28 5°C, in 0.5 M potassium phosphate buffer, pH 7.0, 20% glycerol, 1 mM pyridoxal phosphate and 19 mM 2-mercaptoethanol, stable for more than 10 months Micrococcus percitreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.28 2,4-Dibromo-DL-Tyr 75% of the activity with L-Trp Micrococcus percitreus ?
-
 ?
4.1.1.28 2-Chloro-L-Tyr 77% of the activity with L-Trp Micrococcus percitreus ?
-
 ?
4.1.1.28 2-Methyl-L-Tyr 75% of the activity with L-Trp Micrococcus percitreus ?
-
 ?
4.1.1.28 3,4-Dibromo-DL-Tyr 10% of the activity with L-Trp Micrococcus percitreus ?
-
 ?
4.1.1.28 3,4-Dihydroxyphenylalanine
-
 Micrococcus percitreus 3-Hydroxytyramine + CO2
-
 ?
4.1.1.28 3,5-Dibromo-DL-Tyr 72% of the activity with L-Trp Micrococcus percitreus ?
-
 ?
4.1.1.28 3-Chloro-L-Tyr 81% of the activity with L-Trp Micrococcus percitreus ?
-
 ?
4.1.1.28 3-Hydroxy-DL-kynurenine 8% of the activity with L-Trp Micrococcus percitreus ?
-
 ?
4.1.1.28 3-Methyl-L-Tyr 80% of the activity with L-Trp Micrococcus percitreus ?
-
 ?
4.1.1.28 5-Fluoro-DL-Trp 75% of the activity with L-Trp Micrococcus percitreus ?
-
 ?
4.1.1.28 5-Hydroxytryptophan 24% of the activity with L-Trp Micrococcus percitreus Serotonin + CO2
-
 ?
4.1.1.28 5-Methyl-DL-Trp 17% of the activity with L-Trp Micrococcus percitreus ?
-
 ?
4.1.1.28 7-Aza-DL-Trp 8% of the activity with L-Trp Micrococcus percitreus ?
-
 ?
4.1.1.28 L-kynurenine 15% of the activity with L-Trp Micrococcus percitreus 3-amino-1-(2-aminophenyl)propan-1-one + CO2
-
 ?
4.1.1.28 L-Phe m-DL-Tyr, 17% of the activity with L-Trp Micrococcus percitreus phenylethylamine + CO2
-
 ?
4.1.1.28 L-Phe o-DL-Tyr, 17% of the activity with L-Trp Micrococcus percitreus phenylethylamine + CO2
-
 ?
4.1.1.28 L-Phe L-Tyr, 120% of the activity with L-Trp Micrococcus percitreus phenylethylamine + CO2
-
 ?
4.1.1.28 L-Trp
-
 Micrococcus percitreus Tryptamine + CO2
-
 ?
4.1.1.28 Tyr 87% of the activity with L-Trp Micrococcus percitreus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.28 dimer 2 * 48000, SDS-PAGE Micrococcus percitreus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.28 additional information
-
 in the presence of high concentrations of pyridoxal phosphate, glycerol or potassium phosphate buffer, pH 7.0, stability against heating increases Micrococcus percitreus
4.1.1.28 30
-
 60 min, stable between pH 6.0 and 7.0 Micrococcus percitreus
4.1.1.28 35
-
 pH 7.0, 5 min, stable until Micrococcus percitreus
4.1.1.28 50
-
 pH 7.0, 5 min, rapid inactivation above Micrococcus percitreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.28 9
-
-
 Micrococcus percitreus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.28 4
-
 30°C, 60 min, about 70% loss of activity Micrococcus percitreus
4.1.1.28 5
-
 30°C, 60 min, about 35% loss of activity Micrococcus percitreus
4.1.1.28 6 7 30°C, 60 min, stable Micrococcus percitreus
4.1.1.28 8
-
 30°C, 60 min, about 20% loss of activity Micrococcus percitreus
4.1.1.28 10
-
 30°C, 60 min, about 40% loss of activity Micrococcus percitreus
4.1.1.28 11
-
 30°C, 60 min, about 85% loss of activity Micrococcus percitreus