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Literature summary extracted from

  • Donovan, W.P.; Kushner, S.R.
    Purification and characterization of orotidine-5'-phosphate decarboxylase from Escherichia coli K-12 (1983), J. Bacteriol., 156, 620-624.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.23 0.006
-
 orotidine 5'-phosphate
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.23 27000
-
 2 * 27000, SDS-PAGE Escherichia coli
4.1.1.23 54000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.23 Escherichia coli
-
 harboring the cloned pyrF gene on the multicopy plasmid pDK26
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.23 220
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.23 Orotidine 5'-phosphate
-
 Escherichia coli UMP + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.23 dimer 2 * 27000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.23 26
-
 pH 7.8, 90 min, 20% loss of activity Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.23 6
-
 26°C, 90 min, complete loss of activity. 35% loss of activity after 90 min in presence of azauridine Escherichia coli
4.1.1.23 7.8
-
 26°C, 90 min, 20% loss of activity Escherichia coli