BRENDA - Enzyme Database

Purification and characterization of arginine decarboxylase from soybean (Glycine max) hypocotyls

Nam, K.H.; Lee, S.H.; Lee, J.; Plant Cell Physiol. 38, 1150-1155 (1997)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.19
Ca2+
100 mM, 90% decrease of activity
Glycine max
4.1.1.19
K+
100 mM, 50% decrease of activity
Glycine max
4.1.1.19
Mg2+
100 mM, 90% decrease of activity
Glycine max
4.1.1.19
Mn2+
100 mM, 90% decrease of activity
Glycine max
4.1.1.19
Na+
10 mM, 20% decrease of activity
Glycine max
4.1.1.19
putrescine
-
Glycine max
4.1.1.19
spermidine
-
Glycine max
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.19
0.0461
-
L-Arg
-
Glycine max
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.19
74000
-
3 * 74000, SDS-PAGE
Glycine max
4.1.1.19
240000
-
gel filtration
Glycine max
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.19
Glycine max
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.19
-
Glycine max
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.19
hypocotyl
-
Glycine max
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.1.19
0.0096
-
-
Glycine max
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.19
L-arginine
-
4094
Glycine max
agmatine + CO2
-
4094
Glycine max
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.1.19
trimer
3 * 74000, SDS-PAGE
Glycine max
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.1.1.19
30
50
-
Glycine max
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.1.1.19
50
-
30 min, stable
Glycine max
4.1.1.19
60
-
10 min, 50% loss of activity
Glycine max
4.1.1.19
70
-
5 min, 95% loss of activity
Glycine max
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.19
8
-
Tris-HCl buffer
Glycine max
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.19
Ca2+
100 mM, 90% decrease of activity
Glycine max
4.1.1.19
K+
100 mM, 50% decrease of activity
Glycine max
4.1.1.19
Mg2+
100 mM, 90% decrease of activity
Glycine max
4.1.1.19
Mn2+
100 mM, 90% decrease of activity
Glycine max
4.1.1.19
Na+
10 mM, 20% decrease of activity
Glycine max
4.1.1.19
putrescine
-
Glycine max
4.1.1.19
spermidine
-
Glycine max
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.19
0.0461
-
L-Arg
-
Glycine max
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.19
74000
-
3 * 74000, SDS-PAGE
Glycine max
4.1.1.19
240000
-
gel filtration
Glycine max
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.19
-
Glycine max
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.19
hypocotyl
-
Glycine max
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.1.19
0.0096
-
-
Glycine max
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.19
L-arginine
-
4094
Glycine max
agmatine + CO2
-
4094
Glycine max
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.1.19
trimer
3 * 74000, SDS-PAGE
Glycine max
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.1.1.19
30
50
-
Glycine max
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.1.1.19
50
-
30 min, stable
Glycine max
4.1.1.19
60
-
10 min, 50% loss of activity
Glycine max
4.1.1.19
70
-
5 min, 95% loss of activity
Glycine max
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.19
8
-
Tris-HCl buffer
Glycine max