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Literature summary extracted from
- Rapid purification of threonyl-tRNA synthetase from Saccharomyces carlsbergensis by affinity elution from phosphocellulose (1978), J. Biochem., 83, 1583-1589.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.1.1.3 | 0.00189 | - |
threonine | - |
Saccharomyces pastorianus |  |
| 6.1.1.3 | 0.0023 | - |
tRNAThr | - |
Saccharomyces pastorianus | |
| 6.1.1.3 | 0.179 | - |
tRNAThr | of yeast | Saccharomyces pastorianus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.1.1.3 | K+ | optimal concentration: 150 mM KCl | Saccharomyces pastorianus | |
| 6.1.1.3 | Mg2+ | optimal concentration: 20 mM MgCl2 | Saccharomyces pastorianus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.3 | 82000 | - |
2 * 82000, SDS-PAGE | Saccharomyces pastorianus |
| 6.1.1.3 | 150000 | - |
gel filtration | Saccharomyces pastorianus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.1.1.3 | Saccharomyces pastorianus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.1.1.3 | - |
Saccharomyces pastorianus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.3 | 0.636 | - |
- |
Saccharomyces pastorianus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.1.1.3 | AMP + diphosphate + L-threonyl-tRNAThr | - |
Saccharomyces pastorianus | ATP + L-threonine + tRNAThr | - |
r | |
| 6.1.1.3 | ATP + L-threonine + tRNAThr | - |
Saccharomyces pastorianus | AMP + diphosphate + L-threonyl-tRNAThr | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 6.1.1.3 | dimer | 2 * 82000, SDS-PAGE | Saccharomyces pastorianus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.1.1.3 | 1.73 | - |
L-threonyl-tRNAThr | - |
Saccharomyces pastorianus | |
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Release 2023.1 (January 2023)
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