BRENDA - Enzyme Database

Arginine decarboxylase from Escherichia coli: Arginine decarboxylase from escherichia coli. I. Purification and specificity for substrates and coenzyme

Blethen, S.L.; Boeker, E.A.; Snell, E.E.; J. Biol. Chem. 243, 1671-1677 (1968)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.19
2-Norpyridoxal phosphate
reactivation of the apoenzyme
Escherichia coli
4.1.1.19
6-Methylpyridoxal phosphate
reactivation of the apoenzyme
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.19
2-Nor-2-butylpyridoxal phosphate
-
Escherichia coli
4.1.1.19
5-Deoxypyridoxal
-
Escherichia coli
4.1.1.19
agmatine
-
Escherichia coli
4.1.1.19
beta-(2-Methyl-3-hydroxy-4-formylpyridine-5)-propionic acid
-
Escherichia coli
4.1.1.19
citrulline
-
Escherichia coli
4.1.1.19
guanidine
-
Escherichia coli
4.1.1.19
Guanidinoacetic acid
-
Escherichia coli
4.1.1.19
L-2-Amino-3-guanidinopropanoate
-
Escherichia coli
4.1.1.19
L-2-amino-5-guanidinopentanoate
-
Escherichia coli
4.1.1.19
L-2-amino-6-guanidinohexanoate
-
Escherichia coli
4.1.1.19
L-2-Chloro-5-guanidinopentanoate
-
Escherichia coli
4.1.1.19
L-2-Hydroxy-5-guanidinopentanoate
-
Escherichia coli
4.1.1.19
L-Lys
-
Escherichia coli
4.1.1.19
methylguanidine
-
Escherichia coli
4.1.1.19
Orn
L-Orn
Escherichia coli
4.1.1.19
pyridoxal
-
Escherichia coli
4.1.1.19
spermidine
-
Escherichia coli
4.1.1.19
Urea
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.19
0.65
-
L-Arg
-
Escherichia coli
4.1.1.19
1.2
-
L-canavanine
-
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.19
Escherichia coli
-
-
-
4.1.1.19
Escherichia coli B / ATCC 11303
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.19
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.1.19
350
370
-
Escherichia coli
4.1.1.19
410
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.19
L-arginine
-
4086
Escherichia coli
agmatine + CO2
-
4086
Escherichia coli
-
4.1.1.19
L-arginine
-
4086
Escherichia coli B / ATCC 11303
agmatine + CO2
-
4086
Escherichia coli B / ATCC 11303
-
4.1.1.19
L-Canavanine
-
4086
Escherichia coli
?
-
-
-
-
4.1.1.19
L-Canavanine
-
4086
Escherichia coli B / ATCC 11303
?
-
-
-
-
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.1.1.19
37
40
-
Escherichia coli
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4.1.1.19
20
50
20C: about 40% of maximal activity, 50C: about 70% of maximal activity
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.19
5.2
-
-
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.19
3.5
7
pH 3.5: about 50% of maximal activity, pH 7.0: 23% of maximal activity
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.19
pyridoxal 5'-phosphate
a formyl group of pyridoxal 5'-phosphate is bound to the 6-amino group of a Lys residue. Binds 10 mol of pyridoxal 5'-phosphate per mol of enzyme
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.19
2-Norpyridoxal phosphate
reactivation of the apoenzyme
Escherichia coli
4.1.1.19
6-Methylpyridoxal phosphate
reactivation of the apoenzyme
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.19
pyridoxal 5'-phosphate
a formyl group of pyridoxal 5'-phosphate is bound to the 6-amino group of a Lys residue. Binds 10 mol of pyridoxal 5'-phosphate per mol of enzyme
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.19
2-Nor-2-butylpyridoxal phosphate
-
Escherichia coli
4.1.1.19
5-Deoxypyridoxal
-
Escherichia coli
4.1.1.19
agmatine
-
Escherichia coli
4.1.1.19
beta-(2-Methyl-3-hydroxy-4-formylpyridine-5)-propionic acid
-
Escherichia coli
4.1.1.19
citrulline
-
Escherichia coli
4.1.1.19
guanidine
-
Escherichia coli
4.1.1.19
Guanidinoacetic acid
-
Escherichia coli
4.1.1.19
L-2-Amino-3-guanidinopropanoate
-
Escherichia coli
4.1.1.19
L-2-amino-5-guanidinopentanoate
-
Escherichia coli
4.1.1.19
L-2-amino-6-guanidinohexanoate
-
Escherichia coli
4.1.1.19
L-2-Chloro-5-guanidinopentanoate
-
Escherichia coli
4.1.1.19
L-2-Hydroxy-5-guanidinopentanoate
-
Escherichia coli
4.1.1.19
L-Lys
-
Escherichia coli
4.1.1.19
methylguanidine
-
Escherichia coli
4.1.1.19
Orn
L-Orn
Escherichia coli
4.1.1.19
pyridoxal
-
Escherichia coli
4.1.1.19
spermidine
-
Escherichia coli
4.1.1.19
Urea
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.19
0.65
-
L-Arg
-
Escherichia coli
4.1.1.19
1.2
-
L-canavanine
-
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.19
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.1.19
350
370
-
Escherichia coli
4.1.1.19
410
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.19
L-arginine
-
4086
Escherichia coli
agmatine + CO2
-
4086
Escherichia coli
-
4.1.1.19
L-arginine
-
4086
Escherichia coli B / ATCC 11303
agmatine + CO2
-
4086
Escherichia coli B / ATCC 11303
-
4.1.1.19
L-Canavanine
-
4086
Escherichia coli
?
-
-
-
-
4.1.1.19
L-Canavanine
-
4086
Escherichia coli B / ATCC 11303
?
-
-
-
-
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.1.1.19
37
40
-
Escherichia coli
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4.1.1.19
20
50
20C: about 40% of maximal activity, 50C: about 70% of maximal activity
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.19
5.2
-
-
Escherichia coli
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.19
3.5
7
pH 3.5: about 50% of maximal activity, pH 7.0: 23% of maximal activity
Escherichia coli