BRENDA - Enzyme Database

Arginine decarboxylase from Lathyrus sativus seedlings. Purification and properties

Ramakrishna, S.; Adiga, P.R.; Eur. J. Biochem. 59, 377-386 (1975)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
4.1.1.19
dialysis against Cys causes irreversible inactivation
Lathyrus sativus
4.1.1.19
freeze-thawing or lyophilization inactivates
Lathyrus sativus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.19
4-Bromo-3-hydroxybenzoyloxyamine dihydrogen phosphate
-
Lathyrus sativus
4.1.1.19
agmatine
-
Lathyrus sativus
4.1.1.19
Arcain
-
Lathyrus sativus
4.1.1.19
Ca2+
-
Lathyrus sativus
4.1.1.19
canavanine
-
Lathyrus sativus
4.1.1.19
citrulline
-
Lathyrus sativus
4.1.1.19
Co2+
-
Lathyrus sativus
4.1.1.19
Cu2+
-
Lathyrus sativus
4.1.1.19
D-Arg
-
Lathyrus sativus
4.1.1.19
Hg2+
-
Lathyrus sativus
4.1.1.19
iodoacetamide
-
Lathyrus sativus
4.1.1.19
Mn2+
0.01-0.1 mM, enhances activity. Inhibition at higher concentrations
Lathyrus sativus
4.1.1.19
N-Carbamoylputrescine
-
Lathyrus sativus
4.1.1.19
NaBH4
-
Lathyrus sativus
4.1.1.19
NEM
-
Lathyrus sativus
4.1.1.19
Orn
L-Orn
Lathyrus sativus
4.1.1.19
p-hydroxymercuribenzoate
-
Lathyrus sativus
4.1.1.19
phenylhydrazine
-
Lathyrus sativus
4.1.1.19
putrescine
-
Lathyrus sativus
4.1.1.19
Semicarbazide
-
Lathyrus sativus
4.1.1.19
spermidine
-
Lathyrus sativus
4.1.1.19
spermine
-
Lathyrus sativus
4.1.1.19
Zn2+
-
Lathyrus sativus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.19
1.73
-
L-Arg
-
Lathyrus sativus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.1.19
cytosol
-
Lathyrus sativus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.19
Mn2+
0.1 mM increases activity by 35%. 0.01-0.1 mM, enhances activity. Inhibition at higher concentrations
Lathyrus sativus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.19
220000
-
gel filtration
Lathyrus sativus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.19
Lathyrus sativus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.19
-
Lathyrus sativus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.19
cotyledon
-
Lathyrus sativus
-
4.1.1.19
seedling
-
Lathyrus sativus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.19
1.495
-
-
Lathyrus sativus
Storage Stability
EC Number
Storage Stability
Organism
4.1.1.19
-20°C, labile, even in presence of 20% glycerol
Lathyrus sativus
4.1.1.19
0-2°C, 70% loss of activity after 5 days
Lathyrus sativus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.19
L-arginine
-
4083
Lathyrus sativus
agmatine + CO2
-
4083
Lathyrus sativus
-
4.1.1.19
L-Asp
2.6% of the activity with L-Arg
4083
Lathyrus sativus
?
-
-
-
-
4.1.1.19
L-Glu
2.1% of the activity with L-Arg
4083
Lathyrus sativus
?
-
-
-
-
4.1.1.19
L-Phe
1.4% of the activity with L-Arg
4083
Lathyrus sativus
phenylethylamine + CO2
-
-
-
-
4.1.1.19
L-Pro
3.8% of the activity with L-Arg
4083
Lathyrus sativus
?
-
-
-
-
4.1.1.19
L-Ser
4.4% of the activity with L-Arg
4083
Lathyrus sativus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.1.19
hexamer
6 * 36000-36500, SDS-PAGE
Lathyrus sativus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.1.19
45
-
-
Lathyrus sativus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.1.1.19
37
45
37°C: about 50% of maximal activity, 45°C: maximal activity
Lathyrus sativus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.19
8.5
-
-
Lathyrus sativus
General Stability (protein specific)
EC Number
General Stability
Organism
4.1.1.19
dialysis against Cys causes irreversible inactivation
Lathyrus sativus
4.1.1.19
freeze-thawing or lyophilization inactivates
Lathyrus sativus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.19
4-Bromo-3-hydroxybenzoyloxyamine dihydrogen phosphate
-
Lathyrus sativus
4.1.1.19
agmatine
-
Lathyrus sativus
4.1.1.19
Arcain
-
Lathyrus sativus
4.1.1.19
Ca2+
-
Lathyrus sativus
4.1.1.19
canavanine
-
Lathyrus sativus
4.1.1.19
citrulline
-
Lathyrus sativus
4.1.1.19
Co2+
-
Lathyrus sativus
4.1.1.19
Cu2+
-
Lathyrus sativus
4.1.1.19
D-Arg
-
Lathyrus sativus
4.1.1.19
Hg2+
-
Lathyrus sativus
4.1.1.19
iodoacetamide
-
Lathyrus sativus
4.1.1.19
Mn2+
0.01-0.1 mM, enhances activity. Inhibition at higher concentrations
Lathyrus sativus
4.1.1.19
N-Carbamoylputrescine
-
Lathyrus sativus
4.1.1.19
NaBH4
-
Lathyrus sativus
4.1.1.19
NEM
-
Lathyrus sativus
4.1.1.19
Orn
L-Orn
Lathyrus sativus
4.1.1.19
p-hydroxymercuribenzoate
-
Lathyrus sativus
4.1.1.19
phenylhydrazine
-
Lathyrus sativus
4.1.1.19
putrescine
-
Lathyrus sativus
4.1.1.19
Semicarbazide
-
Lathyrus sativus
4.1.1.19
spermidine
-
Lathyrus sativus
4.1.1.19
spermine
-
Lathyrus sativus
4.1.1.19
Zn2+
-
Lathyrus sativus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.19
1.73
-
L-Arg
-
Lathyrus sativus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.1.19
cytosol
-
Lathyrus sativus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.19
Mn2+
0.1 mM increases activity by 35%. 0.01-0.1 mM, enhances activity. Inhibition at higher concentrations
Lathyrus sativus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.19
220000
-
gel filtration
Lathyrus sativus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.19
-
Lathyrus sativus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.19
cotyledon
-
Lathyrus sativus
-
4.1.1.19
seedling
-
Lathyrus sativus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.19
1.495
-
-
Lathyrus sativus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.1.19
-20°C, labile, even in presence of 20% glycerol
Lathyrus sativus
4.1.1.19
0-2°C, 70% loss of activity after 5 days
Lathyrus sativus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.19
L-arginine
-
4083
Lathyrus sativus
agmatine + CO2
-
4083
Lathyrus sativus
-
4.1.1.19
L-Asp
2.6% of the activity with L-Arg
4083
Lathyrus sativus
?
-
-
-
-
4.1.1.19
L-Glu
2.1% of the activity with L-Arg
4083
Lathyrus sativus
?
-
-
-
-
4.1.1.19
L-Phe
1.4% of the activity with L-Arg
4083
Lathyrus sativus
phenylethylamine + CO2
-
-
-
-
4.1.1.19
L-Pro
3.8% of the activity with L-Arg
4083
Lathyrus sativus
?
-
-
-
-
4.1.1.19
L-Ser
4.4% of the activity with L-Arg
4083
Lathyrus sativus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.1.19
hexamer
6 * 36000-36500, SDS-PAGE
Lathyrus sativus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.1.19
45
-
-
Lathyrus sativus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.1.1.19
37
45
37°C: about 50% of maximal activity, 45°C: maximal activity
Lathyrus sativus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.19
8.5
-
-
Lathyrus sativus