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Literature summary extracted from

  • Ramakrishna, S.; Adiga, P.R.
    Arginine decarboxylase from Lathyrus sativus seedlings. Purification and properties (1975), Eur. J. Biochem., 59, 377-386.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.1.19 dialysis against Cys causes irreversible inactivation Lathyrus sativus
4.1.1.19 freeze-thawing or lyophilization inactivates Lathyrus sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.19 4-Bromo-3-hydroxybenzoyloxyamine dihydrogen phosphate
-
 Lathyrus sativus
4.1.1.19 agmatine
-
 Lathyrus sativus
4.1.1.19 Arcain
-
 Lathyrus sativus
4.1.1.19 Ca2+
-
 Lathyrus sativus
4.1.1.19 canavanine
-
 Lathyrus sativus
4.1.1.19 citrulline
-
 Lathyrus sativus
4.1.1.19 Co2+
-
 Lathyrus sativus
4.1.1.19 Cu2+
-
 Lathyrus sativus
4.1.1.19 D-Arg
-
 Lathyrus sativus
4.1.1.19 Hg2+
-
 Lathyrus sativus
4.1.1.19 iodoacetamide
-
 Lathyrus sativus
4.1.1.19 Mn2+ 0.01-0.1 mM, enhances activity. Inhibition at higher concentrations Lathyrus sativus
4.1.1.19 N-Carbamoylputrescine
-
 Lathyrus sativus
4.1.1.19 NaBH4
-
 Lathyrus sativus
4.1.1.19 NEM
-
 Lathyrus sativus
4.1.1.19 Orn L-Orn Lathyrus sativus
4.1.1.19 p-hydroxymercuribenzoate
-
 Lathyrus sativus
4.1.1.19 phenylhydrazine
-
 Lathyrus sativus
4.1.1.19 putrescine
-
 Lathyrus sativus
4.1.1.19 Semicarbazide
-
 Lathyrus sativus
4.1.1.19 spermidine
-
 Lathyrus sativus
4.1.1.19 spermine
-
 Lathyrus sativus
4.1.1.19 Zn2+
-
 Lathyrus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.19 1.73
-
 L-Arg
-
 Lathyrus sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.19 cytosol
-
 Lathyrus sativus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.19 Mn2+ 0.1 mM increases activity by 35%. 0.01-0.1 mM, enhances activity. Inhibition at higher concentrations Lathyrus sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.19 220000
-
 gel filtration Lathyrus sativus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.19 Lathyrus sativus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.19
-
 Lathyrus sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.19 cotyledon
-
 Lathyrus sativus
-
4.1.1.19 seedling
-
 Lathyrus sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.19 1.495
-
-
 Lathyrus sativus

Storage Stability

EC Number Storage Stability Organism
4.1.1.19 -20°C, labile, even in presence of 20% glycerol Lathyrus sativus
4.1.1.19 0-2°C, 70% loss of activity after 5 days Lathyrus sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.19 L-arginine
-
 Lathyrus sativus agmatine + CO2
-
 ?
4.1.1.19 L-Asp 2.6% of the activity with L-Arg Lathyrus sativus ?
-
 ?
4.1.1.19 L-Glu 2.1% of the activity with L-Arg Lathyrus sativus ?
-
 ?
4.1.1.19 L-Phe 1.4% of the activity with L-Arg Lathyrus sativus phenylethylamine + CO2
-
 ?
4.1.1.19 L-Pro 3.8% of the activity with L-Arg Lathyrus sativus ?
-
 ?
4.1.1.19 L-Ser 4.4% of the activity with L-Arg Lathyrus sativus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.19 hexamer 6 * 36000-36500, SDS-PAGE Lathyrus sativus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.19 45
-
-
 Lathyrus sativus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.19 37 45 37°C: about 50% of maximal activity, 45°C: maximal activity Lathyrus sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.19 8.5
-
-
 Lathyrus sativus