BRENDA - Enzyme Database

Partial purification and characterization of arginine decarboxylase from avocado fruit, a thermostable enzyme

Winer, L.; Vinkler, C.; Apelbaum, A.; Plant Physiol. 76, 233-237 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.19
Cu2+
1 mM, 25% inhibition. 5 mM, 94% inhibition
Persea americana
4.1.1.19
Fluorescein isothiocyanate
0.09 mM, 40% inhibition
Persea americana
4.1.1.19
NEM
3 mM, 65% inhibition
Persea americana
4.1.1.19
Orn
-
Persea americana
4.1.1.19
phosphate
-
Persea americana
4.1.1.19
spermine
1 mM, 5% inhibition of the enzyme from fully developed fruit, 65% inhibition of the enzyme from the seed coat of 4-week-old fruitlets
Persea americana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.19
1.85
-
L-Arg
enzyme from seed coat of 4-week-old fruitlets
Persea americana
4.1.1.19
2.85
-
L-Arg
enzyme from fully developed fruit
Persea americana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.1.19
L-Arg
Persea americana
key enzyme in polyamine metabolism of plants
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.19
Persea americana
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.19
-
Persea americana
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.19
fruit
-
Persea americana
-
4.1.1.19
seed coat
of both 4-week-old avocado fruitlet and fully developed fruit
Persea americana
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.1.19
0.0023
-
-
Persea americana
Storage Stability
EC Number
Storage Stability
Organism
4.1.1.19
-20C, about 30% loss of activity after 60 d
Persea americana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.19
L-Arg
key enzyme in polyamine metabolism of plants
4073
Persea americana
?
-
-
-
-
4.1.1.19
L-arginine
-
4073
Persea americana
agmatine + CO2
-
4073
Persea americana
-
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.1.1.19
60
-
2fold increase in activity after preincubation at 55C for 10 min
Persea americana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.19
8
9
at 60C
Persea americana
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.19
7
11
pH 7: about 25% of maximal activity, pH 11: 80% of maximal activity
Persea americana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.19
Cu2+
1 mM, 25% inhibition. 5 mM, 94% inhibition
Persea americana
4.1.1.19
Fluorescein isothiocyanate
0.09 mM, 40% inhibition
Persea americana
4.1.1.19
NEM
3 mM, 65% inhibition
Persea americana
4.1.1.19
Orn
-
Persea americana
4.1.1.19
phosphate
-
Persea americana
4.1.1.19
spermine
1 mM, 5% inhibition of the enzyme from fully developed fruit, 65% inhibition of the enzyme from the seed coat of 4-week-old fruitlets
Persea americana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.19
1.85
-
L-Arg
enzyme from seed coat of 4-week-old fruitlets
Persea americana
4.1.1.19
2.85
-
L-Arg
enzyme from fully developed fruit
Persea americana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.1.19
L-Arg
Persea americana
key enzyme in polyamine metabolism of plants
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.19
-
Persea americana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.19
fruit
-
Persea americana
-
4.1.1.19
seed coat
of both 4-week-old avocado fruitlet and fully developed fruit
Persea americana
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.1.19
0.0023
-
-
Persea americana
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.1.19
-20C, about 30% loss of activity after 60 d
Persea americana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.19
L-Arg
key enzyme in polyamine metabolism of plants
4073
Persea americana
?
-
-
-
-
4.1.1.19
L-arginine
-
4073
Persea americana
agmatine + CO2
-
4073
Persea americana
-
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.1.1.19
60
-
2fold increase in activity after preincubation at 55C for 10 min
Persea americana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.19
8
9
at 60C
Persea americana
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.19
7
11
pH 7: about 25% of maximal activity, pH 11: 80% of maximal activity
Persea americana