EC Number | General Stability | Organism |
---|---|---|
6.1.1.1 | Tyrosine and/or ATP stabilize | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.1 | tRNATyr | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.1 | additional information | - |
additional information | values for mutant strains | Escherichia coli | |
6.1.1.1 | 0.0002 | - |
L-tyrosyl-tRNATyr | deacylation | Escherichia coli | |
6.1.1.1 | 0.00052 | - |
tRNATyr | acylation | Escherichia coli | |
6.1.1.1 | 0.008 | - |
tyrosine | acylation | Escherichia coli | |
6.1.1.1 | 0.012 | - |
tyrosine | ATP-diphosphate exchange reaction | Escherichia coli | |
6.1.1.1 | 0.5 | - |
ATP | ATP-diphosphate exchange reaction | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.1.1.1 | soluble | - |
Escherichia coli | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.1.1.1 | 90000 | - |
sucrose density gradient centrifugation | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.1 | Escherichia coli | - |
- |
- |
EC Number | Storage Stability | Organism |
---|---|---|
6.1.1.1 | 4°C, 20 mM Tris-HCl buffer, pH 7, 80% loss of activity within 48 h, 5% loss of activity in presence of 10% glycerol and 3 mM 2-mercaptoethanol | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.1 | AMP + diphosphate | - |
Escherichia coli | ATP | - |
? | |
6.1.1.1 | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
Escherichia coli | ATP + L-tyrosine + tRNATyr | - |
? | |
6.1.1.1 | ATP + tyrosine + tRNATyr | r | Escherichia coli | AMP + Tyr-tRNATyr + diphosphate | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.1 | 46 | - |
40% loss of activity in 4-12 min, depending on the bacterial strains used for purification, stabilization by substrates | Escherichia coli |