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Literature summary extracted from

  • Zheltonosova, J.; Melnikova, E.; Garber, M.; Reinbolt, J.; Kern, D.; Ehresmann, C.; Ehresmann, B.
    Threonyl-tRNA synthetase from Thermus thermophilus. Purification and some structural and kinetic properties (1994), Biochimie, 76, 71-77.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.3 0.000037 0.00007 tRNAThr of Thermus thermophilus, , depending on temperature Thermus thermophilus
6.1.1.3 0.00005
-
 tRNAThr of E. coli Escherichia coli
6.1.1.3 0.000085
-
 tRNAThr
-
 Escherichia coli
6.1.1.3 0.00014
-
 tRNAThr of E. coli Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.3 77000
-
 2 * 77000, SDS-PAGE under denaturing conditions Thermus thermophilus
6.1.1.3 150000
-
-
 Escherichia coli
6.1.1.3 150000
-
 gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.3 Escherichia coli
-
-
-
6.1.1.3 Thermus thermophilus
-
-
-
6.1.1.3 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.3
-
 Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.3 0.353
-
-
 Thermus thermophilus

Storage Stability

EC Number Storage Stability Organism
6.1.1.3 4°C Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNAThr
-
 Thermus thermophilus AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
6.1.1.3 ATP + L-threonine + tRNAThr
-
 Escherichia coli AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
6.1.1.3 ATP + L-threonine + tRNAThr tRNAThr of Thermus thermophilus Thermus thermophilus AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
6.1.1.3 ATP + L-threonine + tRNAThr tRNAThr3 of Escherichia coli Escherichia coli AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
6.1.1.3 ATP + L-threonine + tRNAThr
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
6.1.1.3 ATP + L-threonine + tRNAThr tRNAThr of Thermus thermophilus Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-threonyl-tRNAThr
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.3 dimer
-
 Escherichia coli
6.1.1.3 dimer 2 * 77000, SDS-PAGE under denaturing conditions Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.3 37
-
-
 Escherichia coli
6.1.1.3 70
-
-
 Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.3 0.037 0.23 tRNAThr
-
 Thermus thermophilus
6.1.1.3 0.21
-
 tRNAThr tRNAThr of E. coli Thermus thermophilus
6.1.1.3 0.52 0.7 tRNAThr tRNAThr of Thermus thermophilus Escherichia coli
6.1.1.3 0.53
-
 tRNAThr tRNAThr of E. coli Escherichia coli