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Literature summary extracted from

  • Nozaki, M.
    Aromatic amine dehydrogenase from Alcaligenes faecalis (1987), Methods Enzymol., 142, 650-655.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.9.2
-
 Alcaligenes faecalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.9.2 hydroxylamine
-
 Alcaligenes faecalis
1.4.9.2 phenylhydrazine
-
 Alcaligenes faecalis
1.4.9.2 Semicarbazide
-
 Alcaligenes faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.9.2 8000
-
 alpha2beta2, 2 * 46000 + 2 * 8000, high speed gel filtration in 6 M guanidine hydrochloride Alcaligenes faecalis
1.4.9.2 46000
-
 alpha2beta2, 2 * 46000 + 2 * 8000, high speed gel filtration in 6 M guanidine hydrochloride Alcaligenes faecalis
1.4.9.2 103000
-
 sedimentation equilibrium Alcaligenes faecalis

Organism

EC Number Organism UniProt Comment Textmining
1.4.9.2 Alcaligenes faecalis
-
-
-
1.4.9.2 Alcaligenes faecalis IFO 14479
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.9.2
-
 Alcaligenes faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.9.2 9.11
-
 after purification Alcaligenes faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.9.2 dopamine + acceptor + H2O
-
 Alcaligenes faecalis 4-(2-oxoethyl)-1,2-benzenediol + NH3 + reduced acceptor
-
 ?
1.4.9.2 dopamine + acceptor + H2O
-
 Alcaligenes faecalis IFO 14479 4-(2-oxoethyl)-1,2-benzenediol + NH3 + reduced acceptor
-
 ?
1.4.9.2 additional information slow reaction with aromatic amines Alcaligenes faecalis ?
-
 ?
1.4.9.2 additional information slow reaction with aromatic amines Alcaligenes faecalis IFO 14479 ?
-
 ?
1.4.9.2 serotonin + acceptor + H2O
-
 Alcaligenes faecalis 3-(2-oxoethyl)-1H-indol-5-ol + NH3 + reduced acceptor
-
 ?
1.4.9.2 serotonin + acceptor + H2O
-
 Alcaligenes faecalis IFO 14479 3-(2-oxoethyl)-1H-indol-5-ol + NH3 + reduced acceptor
-
 ?
1.4.9.2 tryptamine + acceptor + H2O
-
 Alcaligenes faecalis 1H-indole-3-acetaldehyde + NH3 + reduced acceptor
-
 ?
1.4.9.2 tryptamine + acceptor + H2O
-
 Alcaligenes faecalis IFO 14479 1H-indole-3-acetaldehyde + NH3 + reduced acceptor
-
 ?
1.4.9.2 tyramine + acceptor + H2O
-
 Alcaligenes faecalis 4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
 ?
1.4.9.2 tyramine + acceptor + H2O
-
 Alcaligenes faecalis IFO 14479 4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.9.2 tetramer alpha2beta2, 2 * 46000 + 2 * 8000, high speed gel filtration in 6 M guanidine hydrochloride Alcaligenes faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.9.2 25
-
 assay at Alcaligenes faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.9.2 additional information probably chromophore with quinone structure Alcaligenes faecalis