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Literature summary extracted from

  • Zhu, X.; Tang, G.; Galili, G.
    Characterization of the two saccharopine dehydrogenase isozymes of lysine catabolism encoded by the single composite AtLKR/SDH locus of Arabidopsis (2000), Plant Physiol., 124, 1363-1371.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.9 in yeast for heterologous expression Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.9 0.035
-
saccharopine pH 9, bifunctional enzyme, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana
1.5.1.9 0.05
-
saccharopine pH 9, monofunctional enzyme Arabidopsis thaliana
1.5.1.9 0.063
-
saccharopine pH 7, bifunctional enzyme, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana
1.5.1.9 0.13
-
saccharopine pH 7, monofunctional enzyme Arabidopsis thaliana
1.5.1.9 0.333
-
NAD+ pH 7, monofunctional enzyme Arabidopsis thaliana
1.5.1.9 0.374
-
NAD+ pH 7, bifunctional enzyme, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana
1.5.1.9 0.698
-
NAD+ pH 9, bifunctional enzyme, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana
1.5.1.9 0.759
-
NAD+ pH 9, monofunctional enzyme Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.9 cytosol
-
Arabidopsis thaliana 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.9 63000
-
x * 63000, SDS-PAGE, monofunctional enzyme Arabidopsis thaliana
1.5.1.9 120000
-
x * 120000, SDS-PAGE, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Arabidopsis thaliana involved in lysine catabolism L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.9 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.9 heterologously expressed enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
Arabidopsis thaliana L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
r
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O involved in lysine catabolism Arabidopsis thaliana L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.5.1.9 ? x * 63000, SDS-PAGE, monofunctional enzyme Arabidopsis thaliana
1.5.1.9 ? x * 120000, SDS-PAGE, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.5.1.9 AtLKR/SDHp bifunctional enzyme, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana
1.5.1.9 AtSDHp monofunctional enzyme Arabidopsis thaliana
1.5.1.9 SDH
-
Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.9 9
-
monofunctional enzyme Arabidopsis thaliana
1.5.1.9 9.5
-
bifunctional enzyme, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.9 NAD+
-
Arabidopsis thaliana