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Literature summary extracted from

  • Rao, P.V.S.; Vaidyanathan, C.S.
    Studies on the metabolism of o-aminophenol. Purification and properties of isophenoxazine synthase from Bauhenia monandra (1967), Arch. Biochem. Biophys., 118, 388-394.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.10.3.4 stable to extensive dialysis against cyanide or EDTA Bauhinia monandra

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.4 2,3-Dimercaptopropanol
-
 Bauhinia monandra
1.10.3.4 3-hydroxyanthranilic acid competitive Bauhinia monandra
1.10.3.4 Ag+
-
 Bauhinia monandra
1.10.3.4 anthranilic acid competitive Bauhinia monandra
1.10.3.4 ascorbic acid
-
 Bauhinia monandra
1.10.3.4 Atebrin slightly inhibitory Bauhinia monandra
1.10.3.4 azide
-
 Bauhinia monandra
1.10.3.4 Cu2+
-
 Bauhinia monandra
1.10.3.4 cyanide
-
 Bauhinia monandra
1.10.3.4 cysteine
-
 Bauhinia monandra
1.10.3.4 Fe2+
-
 Bauhinia monandra
1.10.3.4 glutathione
-
 Bauhinia monandra
1.10.3.4 Hg2+
-
 Bauhinia monandra
1.10.3.4 N-ethylmaleimide no effect Bauhinia monandra
1.10.3.4 p-hydroxymercuribenzoate reversed by glutathione or cysteine Bauhinia monandra

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.4 0.75
-
 o-aminophenol
-
 Bauhinia monandra

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.4 additional information no metal requirement Bauhinia monandra

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.4 additional information Bauhinia monandra involved in biosynthesis of catechol ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.4 Bauhinia monandra
-
 leguminous plant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.4
-
 Bauhinia monandra

Reaction

EC Number Reaction Comment Organism Reaction ID
1.10.3.4 4 2-aminophenol + 3 O2 = 2 2-aminophenoxazin-3-one + 6 H2O discussion of mechanism, isophenoxazine may be formed by a secondary condensation from the initial oxidation product Bauhinia monandra
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.4 56.1
-
-
 Bauhinia monandra

Storage Stability

EC Number Storage Stability Organism
1.10.3.4 -20°C, stable for at least 1 month Bauhinia monandra

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.4 2 2-aminophenol + 2 O2 highly specific for o-aminophenol, not: related compounds (e.g. 3-hydroxyanthranilic acid, 3-hydroxy-kynurenine, p-aminophenol, catechol) Bauhinia monandra isophenoxazine + 2 H2O + H2O2
-
 ?
1.10.3.4 additional information involved in biosynthesis of catechol Bauhinia monandra ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.4 40
-
-
 Bauhinia monandra

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.4 6.2
-
-
 Bauhinia monandra

Cofactor

EC Number Cofactor Comment Organism Structure
1.10.3.4 additional information no cofactor requirement Bauhinia monandra