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Literature summary extracted from

  • Saito, K.; Komamine, A.
    Biosynthesis of stizolobinic acid and stizolobic acid in higher plants. An enzyme system(s) catalyzing the conversion of dihydroxyphenylalanine into stizolobinic acid and stizolobic acid from etiolated seedlings of Stizolobium hassjoo (1976), Eur. J. Biochem., 68, 237-243.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.29 3,4-dihydroxy-L-phenylalanine + O2 Stizolobium hassjoo
-
4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
?
1.13.11.30 3,4-dihydroxy-L-phenylalanine + O2 Stizolobium hassjoo
-
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.29 Stizolobium hassjoo
-
-
-
1.13.11.30 Stizolobium hassjoo
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.29 seedling etiolated Stizolobium hassjoo
-
1.13.11.30 seedling etiolated Stizolobium hassjoo
-

Storage Stability

EC Number Storage Stability Organism
1.13.11.29 storage under nitrogen stabilizes Stizolobium hassjoo

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.29 3,4-dihydroxy-L-phenylalanine + O2
-
Stizolobium hassjoo 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
?
1.13.11.29 3,4-dihydroxy-L-phenylalanine + O2
-
Stizolobium hassjoo 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde undergoes ring closure and oxidation to stizolobate with NAD(P)+ as acceptor ?
1.13.11.30 3,4-dihydroxy-L-phenylalanine + O2
-
Stizolobium hassjoo 5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
?
1.13.11.30 3,4-dihydroxy-L-phenylalanine + O2
-
Stizolobium hassjoo 5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde undergoes ring closure and oxidation to stizolobinate with NAD(P)+ as acceptor ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.29 7.4
-
-
Stizolobium hassjoo

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.29 NADP+ required for reaction Stizolobium hassjoo
1.13.11.29 NAD+ required for reaction, less efficient than NADP+ Stizolobium hassjoo
1.13.11.30 NAD+
-
Stizolobium hassjoo
1.13.11.30 NADP+ required for reaction Stizolobium hassjoo