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Literature summary extracted from

  • Wood, D.A.
    Production, purification and properties of extracellular laccase of Agaricus bisporus (1980), J. Gen. Microbiol., 117, 327-338.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 azide
-
 Agaricus bisporus
1.10.3.2 Cetylpyridinium bromide
-
 Agaricus bisporus
1.10.3.2 cetyltrimethylammonium bromide
-
 Agaricus bisporus
1.10.3.2 CN-
-
 Agaricus bisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.1
-
 4-phenylenediamine
-
 Agaricus bisporus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Agaricus bisporus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 copper 1.8 gatom per mol Agaricus bisporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 96000 110000 sucrose density gradient centrifugation, gel filtration Agaricus bisporus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Agaricus bisporus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein sugar content 15% Agaricus bisporus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Agaricus bisporus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 culture filtrate
-
 Agaricus bisporus
-

Storage Stability

EC Number Storage Stability Organism
1.10.3.2 -20°C, 0.01 M phosphate buffer, pH 7.0, at least 1 year Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 1-naphthol + O2
-
 Agaricus bisporus ? + H2O
-
 ?
1.10.3.2 2 catechol + O2
-
 Agaricus bisporus 2 6-hydroxycyclohexa-2,4-dienone + 2 H2O
-
 ?
1.10.3.2 4-phenylenediamine + O2
-
 Agaricus bisporus ?
-
 r
1.10.3.2 ascorbate + O2
-
 Agaricus bisporus ?
-
 ?
1.10.3.2 gallic acid + O2
-
 Agaricus bisporus ?
-
 ?
1.10.3.2 additional information overview Agaricus bisporus ?
-
 ?
1.10.3.2 additional information diphenols not substrates Agaricus bisporus ?
-
 ?
1.10.3.2 pyrogallol + O2
-
 Agaricus bisporus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 oligomer x * 21400-56000, SDS-PAGE Agaricus bisporus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 50
-
 half-life 3 h Agaricus bisporus
1.10.3.2 60
-
 half-life 40 min Agaricus bisporus
1.10.3.2 70
-
 half-life 10 min Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3.5
-
 substrate p-phenylenediamine, has two optima: pH 3.5 and 5.6 Agaricus bisporus
1.10.3.2 5.6
-
 substrate p-phenylenediamine, has two optima: pH 3.5 and 5.6 Agaricus bisporus
1.10.3.2 5.6
-
 substrate guaiacol Agaricus bisporus
1.10.3.2 5.6
-
 substrate N,N'-dimethyl-p-phenylenediamine Agaricus bisporus