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Literature summary extracted from

  • Rogalski, J.; Wojtas-Wasilewska, M.; Apalovic, R.; Leonowicz, A.
    Affinity chromatography: a convenient method for purification of fungal lactases (1991), Biotechnol. Bioeng., 37, 770-777.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 additional information
-
 additional information comparison of values for free and immobilized laccase Fomes fomentarius
1.10.3.2 0.03
-
 syringic acid immobilized enzyme Fomes fomentarius
1.10.3.2 0.055
-
 syringic acid immobilized enzyme Fomes fomentarius
1.10.3.2 0.06
-
 vanillic acid immobilized enzyme Trametes versicolor
1.10.3.2 0.13
-
 syringic acid
-
 Trametes versicolor
1.10.3.2 0.28
-
 vanillic acid
-
 Trametes versicolor
1.10.3.2 0.28
-
 syringic acid
-
 Fomes fomentarius
1.10.3.2 0.97
-
 vanillic acid immobilized enzyme Fomes fomentarius
1.10.3.2 2.92
-
 vanillic acid
-
 Fomes fomentarius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Trametes versicolor
-
-
1.10.3.2 extracellular
-
 Fomes fomentarius
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Fomes fomentarius
-
-
-
1.10.3.2 Trametes versicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Trametes versicolor
1.10.3.2
-
 Fomes fomentarius

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 culture filtrate
-
 Trametes versicolor
-
1.10.3.2 culture filtrate
-
 Fomes fomentarius
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 additional information
-
 depending on isozyme, substrate and pH Trametes versicolor
1.10.3.2 additional information
-
 depending on isozyme, substrate and pH Fomes fomentarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 4 syringic acid + O2
-
 Trametes versicolor 4 4-carboxy-2,6-dimethoxyphenoxyl + 2 H2O
-
 ?
1.10.3.2 4 syringic acid + O2
-
 Fomes fomentarius 4 4-carboxy-2,6-dimethoxyphenoxyl + 2 H2O
-
 ?
1.10.3.2 ferulic acid + O2
-
 Trametes versicolor 3-(4-hydroxy-3-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
 ?
1.10.3.2 ferulic acid + O2
-
 Fomes fomentarius 3-(4-hydroxy-3-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
 ?
1.10.3.2 sinapic acid + O2
-
 Trametes versicolor 3-(3,5-dimethoxy-4-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
 ?
1.10.3.2 sinapic acid + O2
-
 Fomes fomentarius 3-(3,5-dimethoxy-4-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
 ?
1.10.3.2 vanillic acid + O2
-
 Trametes versicolor 4-carboxy-2-methoxycyclohexa-2,4-dienone + H2O
-
 ?
1.10.3.2 vanillic acid + O2
-
 Fomes fomentarius 4-carboxy-2-methoxycyclohexa-2,4-dienone + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 38
-
 immobilized Fomes fomentarius
1.10.3.2 52
-
 free enzyme Fomes fomentarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 additional information
-
 dependency on isozyme, substrate and organism Trametes versicolor
1.10.3.2 additional information
-
 dependency on isozyme, substrate and organism Fomes fomentarius
1.10.3.2 3.5
-
 substrate sinapic acid Trametes versicolor
1.10.3.2 3.8
-
 substrate sinapic acid Fomes fomentarius
1.10.3.2 3.8 4.8 depending on substrate and organism Trametes versicolor
1.10.3.2 3.8 4.8 depending on substrate and organism Fomes fomentarius
1.10.3.2 4
-
 substrate ferulic acid Trametes versicolor
1.10.3.2 4.8
-
 substrate ferulic acid Fomes fomentarius