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Literature summary extracted from
- The metabolism of nicotinic acid. I. Purification and properties of 2,5-dihydroxypyridine oxygenase from Pseudomonas putida N-9 (1971), J. Biol. Chem., 246, 3737-3742.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.13.11.9 | - |
Pseudomonas putida |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.13.11.9 | combination of dithiothreitol and FeSO4 is detrimental during long-term incubation at 0°C | Pseudomonas putida |
| 1.13.11.9 | dithiothreitol stabilizes | Pseudomonas putida |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.9 | 1,10-phenanthroline | - |
Pseudomonas putida |  |
| 1.13.11.9 | 2,2'-dipyridyl | - |
Pseudomonas putida | |
| 1.13.11.9 | EDTA | - |
Pseudomonas putida | |
| 1.13.11.9 | H2O2 | - |
Pseudomonas putida | |
| 1.13.11.9 | iodoacetamide | - |
Pseudomonas putida | |
| 1.13.11.9 | KCN | - |
Pseudomonas putida | |
| 1.13.11.9 | N-ethylmaleimide | - |
Pseudomonas putida | |
| 1.13.11.9 | p-chloromercuribenzoate | - |
Pseudomonas putida | |
| 1.13.11.9 | Tiron | i.e. 4,5-dihydroxy-1,3-benzene disulfonic acid | Pseudomonas putida | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.9 | Iron | Fe2+ requirement | Pseudomonas putida | |
| 1.13.11.9 | Iron | enzyme contains loosly bound Fe3+ | Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.9 | 39500 | - |
x * 39500, SDS-PAGE | Pseudomonas putida |
| 1.13.11.9 | 242000 | - |
sucrose density gradient centrifugation (+ dithiothreitol) | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.9 | 2,5-dihydroxypyridine + O2 + H2O | Pseudomonas putida | nicotinic acid catabolism | maleamate + formate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.9 | Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.9 | - |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.9 | 38.3 | - |
- |
Pseudomonas putida |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.13.11.9 | -40°C, 3 months, 20% loss of activity | Pseudomonas putida |
| 1.13.11.9 | 0°C, 20 mM sodium phosphate, pH 7.5, half-life: 2-3 days, addition of dithiothreitol extends half-life to about 2 weeks | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.9 | 2,5-dihydroxypyridine + O2 + H2O | strictly specific for 2,5-dihydroxypyridine | Pseudomonas putida | maleamate + formate | - |
? | |
| 1.13.11.9 | 2,5-dihydroxypyridine + O2 + H2O | nicotinic acid catabolism | Pseudomonas putida | maleamate + formate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.9 | ? | x * 39500, SDS-PAGE | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.9 | 8 | - |
- |
Pseudomonas putida |
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