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Literature summary extracted from

  • White, G.A.; Krupka, R.M.
    Ascorbic acid oxidase and ascorbic acid oxygenase of Myrothecium verrucaria (1965), Arch. Biochem. Biophys., 110, 448-461.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.3 2,2'-bipyridyl stimulation Albifimbria verrucaria
1.10.3.3 ethanol stimulation Albifimbria verrucaria
1.10.3.3 H2O2 stimulation at low concentration, 0.056 mM Albifimbria verrucaria
1.10.3.3 iodoacetate stimulation Albifimbria verrucaria
1.10.3.3 o-phenanthroline stimulation Albifimbria verrucaria
1.10.3.3 p-chloromercuribenzoate stimulation Albifimbria verrucaria

General Stability

EC Number General Stability Organism
1.10.3.3 stable to dialysis against EDTA or cyanide Albifimbria verrucaria

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.3 cyanide very slight Albifimbria verrucaria
1.10.3.3 ethyl xanthate
-
 Albifimbria verrucaria
1.10.3.3 Fenton's reagent Fe2+ + H2O2 + 2 H+ Albifimbria verrucaria
1.10.3.3 H2O2 inhibition at 5.6 mM, stimulation at 0.56 mM Albifimbria verrucaria
1.10.3.3 KNO3
-
 Albifimbria verrucaria
1.10.3.3 Lauryl sulfate
-
 Albifimbria verrucaria
1.10.3.3 additional information reaction inactivation: progressive loss of activity during oxidation of ascorbic acid, not inhibited by diethyldithiocarbamate Albifimbria verrucaria
1.10.3.3 NaNO2
-
 Albifimbria verrucaria
1.10.3.3 Nitrofurantoin slight Albifimbria verrucaria

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.3 additional information no support of a metal in the enzyme Albifimbria verrucaria

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.3 Albifimbria verrucaria
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.3
-
 Albifimbria verrucaria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.3 2 L-ascorbate + O2 highly specific for ascorbic acid and a few of its analogs and O2 Albifimbria verrucaria 2 L-dehydroascorbate + 2 H2O
-
 ?