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Literature summary extracted from

  • Wigley, W.C.; Prihoda, J.S.; Mowszowicz, I.; Mendonca, B.B.; New, M.I.; Wilson, J.D.; Russel, D.W.
    Natural mutagenesis study of the human steroid 5alpha-reductase 2 isozyme (1994), Biochemistry, 33, 1265-1270.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.22 G183S mutant of 5alpha-reductase isozyme type-2 Homo sapiens
1.3.1.22 G196S mutant of 5alpha-reductase isozyme type-2 Homo sapiens
1.3.1.22 G34R mutant of 5alpha-reductase isozyme type-2 Homo sapiens
1.3.1.22 H231R mutant of 5alpha-reductase isozyme type-2 Homo sapiens
1.3.1.22 H264Q mutant of 5alpha-reductase isozyme type-2 Homo sapiens
1.3.1.22 N193S mutant of 5alpha-reductase isozyme type-2 Homo sapiens
1.3.1.22 P181L mutant of 5alpha-reductase isozyme type-2 Homo sapiens
1.3.1.22 R145W mutant of 5alpha-reductase isozyme type-2 Homo sapiens
1.3.1.22 R171S mutant of 5alpha-reductase isozyme type-2 Homo sapiens
1.3.1.22 R246W mutant of 5alpha-reductase isozyme type-2 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.22 0.0009
-
 testosterone enzyme mutant R246Q Homo sapiens
1.3.1.22 0.001
-
 testosterone
-
 Homo sapiens
1.3.1.22 0.001
-
 testosterone enzyme mutant G196S Homo sapiens
1.3.1.22 0.001
-
 testosterone enzyme mutant R246W Homo sapiens
1.3.1.22 0.0012
-
 testosterone enzyme mutant R145W Homo sapiens
1.3.1.22 0.0012
-
 testosterone enzyme mutant N193S Homo sapiens
1.3.1.22 0.0018
-
 testosterone enzyme mutant G183S Homo sapiens
1.3.1.22 0.0022
-
 testosterone enzyme mutant P181L Homo sapiens
1.3.1.22 0.0023
-
 testosterone enzyme mutant R171S Homo sapiens
1.3.1.22 0.012
-
 testosterone enzyme mutant G34R Homo sapiens
1.3.1.22 0.014
-
 testosterone enzyme mutant H231R Homo sapiens
1.3.1.22 0.015
-
 NADPH enzyme mutant G34R Homo sapiens
1.3.1.22 0.02
-
 NADPH
-
 Homo sapiens
1.3.1.22 0.025
-
 NADPH enzyme mutant H231R Homo sapiens
1.3.1.22 0.18
-
 NADPH enzyme mutant G196S Homo sapiens
1.3.1.22 0.316
-
 NADPH enzyme mutant G183S Homo sapiens
1.3.1.22 0.325
-
 NADPH enzyme mutant N193S Homo sapiens
1.3.1.22 0.34
-
 NADPH enzyme mutant R171S Homo sapiens
1.3.1.22 0.401
-
 NADPH enzyme mutant R246Q Homo sapiens
1.3.1.22 0.526
-
 NADPH enzyme mutant P181L Homo sapiens
1.3.1.22 0.584
-
 NADPH enzyme mutant R145W Homo sapiens
1.3.1.22 0.65
-
 NADPH enzyme mutant R246W Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.22 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.22 testosterone + NADPH + H+
-
 Homo sapiens 17beta-hydroxy-5alpha-androstan-3-one + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.22 5
-
-
 Homo sapiens
1.3.1.22 5.2 5.6 mutant G34R Homo sapiens
1.3.1.22 6 6.5 mutant P181L Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.22 NADPH
-
 Homo sapiens