EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.22 | Ca2+ | 1 mM: less than 20% inhibition | Rattus norvegicus | |
1.3.1.22 | Cd2+ | 1 mM: less than 20% inhibition | Rattus norvegicus | |
1.3.1.22 | Cu2+ | 1 mM | Rattus norvegicus | |
1.3.1.22 | Mn2+ | 1 mM: less than 20% inhibition | Rattus norvegicus | |
1.3.1.22 | Zn2+ | 1 mM | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.22 | 0.032 | - |
testosterone | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.1.22 | membrane | - |
Rattus norvegicus | 16020 | - |
1.3.1.22 | nuclear envelope | - |
Rattus norvegicus | 5635 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.22 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.22 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.1.22 | epididymis | - |
Rattus norvegicus | - |
1.3.1.22 | genitalia | external | Rattus norvegicus | - |
1.3.1.22 | additional information | highest activity in organs of acessory reproduction and in liver | Rattus norvegicus | - |
1.3.1.22 | preputial gland | - |
Rattus norvegicus | - |
1.3.1.22 | prostate gland | ventral | Rattus norvegicus | - |
1.3.1.22 | seminal vesicle | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.22 | additional information | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
1.3.1.22 | -20°C, 6 months | Rattus norvegicus |
1.3.1.22 | 4°C, 2 days, 50% loss of activity | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.22 | 17alpha-hydroxyprogesterone + NADPH | - |
Rattus norvegicus | 17alpha-hydroxy-5alpha-pregnan-3,20-dione + NADP+ | - |
ir | |
1.3.1.22 | 20-alpha-hydroxypregn-4-ene-3one + NADPH | - |
Rattus norvegicus | 20-alpha-hydroxy-5alpha-pregnan-3-one + NADP+ | - |
ir | |
1.3.1.22 | androstenediol + NADPH | - |
Rattus norvegicus | 5alpha-androstan-3beta,17beta-diol + NADP+ | - |
ir | |
1.3.1.22 | androstenedione + NADPH + H+ | - |
Rattus norvegicus | 5alpha-androstan-3,17-dione + NADP+ | - |
ir | |
1.3.1.22 | cholest-4-en-3-one + NADPH | mammalian enzymes: no reverse reaction, first step in reverse direction (enolization) occurs, but energy barrier for hydride abstraction is extremely high | Rattus norvegicus | 5-alpha-cholestan-3-one + NADP+ | - |
ir | |
1.3.1.22 | cortexolone + NADPH | - |
Rattus norvegicus | 17alpha,21-dihydroxy-5alpha-pregnan-3,20-dione + NADP+ | - |
ir | |
1.3.1.22 | deoxycorticosterone + NADPH | - |
Rattus norvegicus | 21-hydroxy-5alpha-pregnan-3,20-dione | - |
ir | |
1.3.1.22 | epitestosterone + NADPH | - |
Rattus norvegicus | ? | - |
ir | |
1.3.1.22 | progesterone + NADPH + H+ | - |
Rattus norvegicus | 5alpha-pregnan-3,20-dione + NADP+ | - |
ir | |
1.3.1.22 | testosterone + NADPH + H+ | - |
Rattus norvegicus | 17beta-hydroxy-5alpha-androstan-3-one + NADP+ | - |
ir |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.1.22 | 25 | - |
1 h stable | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.22 | 6.6 | - |
- |
Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.22 | additional information | - |
Rattus norvegicus | |
1.3.1.22 | NADPH | - |
Rattus norvegicus |