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Literature summary extracted from

  • Fukumori, Y.; Nakayama, K.; Yamanaka, T.
    Cytochrome c oxidase of Pseudomonas AM 1: purification, and molecular and enzymatic properties (1985), J. Biochem., 98, 493-499.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.9 CN- 0.003 mM, 50% inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.9 0.0087
-
 ferrocytochrome c Candida krusei cytochrome c Pseudomonas sp.
7.1.1.9 0.05
-
 ferrocytochrome c horse and native cytochrome c Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.9 Cu
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.9 30000
-
 1 * 30000 + 1 * 50000, SDS-PAGE Pseudomonas sp.
7.1.1.9 30000
-
 x * 50000 + x * 30000, SDS-PAGE Pseudomonas sp.
7.1.1.9 50000
-
 1 * 30000 + 1 * 50000, SDS-PAGE Pseudomonas sp.
7.1.1.9 50000
-
 x * 50000 + x * 30000, SDS-PAGE Pseudomonas sp.
7.1.1.9 79000
-
 SDS-PAGE, no mercaptoethanol Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.9 Pseudomonas sp.
-
 AM1
-
7.1.1.9 Pseudomonas sp. AM1
-
 AM1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.9 DEAE-cellulose, Sephacryl S-200 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.9 24
-
 cytochrome c Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.9 ferrocytochrome c + O2 horse ferrocytochrome c Pseudomonas sp. ferricytochrome c + H2O
-
 ?
7.1.1.9 ferrocytochrome c + O2 Candida krusei ferrocytochrome c Pseudomonas sp. ferricytochrome c + H2O
-
 ?
7.1.1.9 ferrocytochrome c + O2 horse ferrocytochrome c Pseudomonas sp. AM1 ferricytochrome c + H2O
-
 ?
7.1.1.9 ferrocytochrome c + O2 Candida krusei ferrocytochrome c Pseudomonas sp. AM1 ferricytochrome c + H2O
-
 ?
7.1.1.9 ferrocytochrome c + O2 + H+
-
 Pseudomonas sp. ferricytochrome c + H2O
-
 r
7.1.1.9 ferrocytochrome c + O2 + H+
-
 Pseudomonas sp. AM1 ferricytochrome c + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
7.1.1.9 ? x * 50000 + x * 30000, SDS-PAGE Pseudomonas sp.
7.1.1.9 dimer 1 * 30000 + 1 * 50000, SDS-PAGE Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.9 91
-
 ferrocytochrome c
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.1.9 6
-
 electron donor: cytochrome c from Candida krusei Pseudomonas sp.
7.1.1.9 6.4
-
 electron donor: cytochrome c from Pseudomonas sp. AM1 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.9 heme a
-
 Pseudomonas sp.