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Literature summary extracted from

  • Bisson, R.; Schiavo, G.; Papini, E.
    Cytochrome c oxidase from the slime mold Dictyostelium discoideum: purification and characterization (1985), Biochemistry, 24, 7845-7852.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.9 0.00126
-
ferrocytochrome c 2 Km values for horse heart, Neurospora crassa, Saccharomyces cerevisiea and Candida krusei cytochrome c Dictyostelium discoideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.9 5700
-
x * 55000 + x * 29500 + x * 19000 + x * 13000 + x * 11000 + x * 5700, homolog to eukaryotic subunit III is lost during purification, SDS-PAGE Dictyostelium discoideum
7.1.1.9 11000
-
x * 55000 + x * 29500 + x * 19000 + x * 13000 + x * 11000 + x * 5700, homolog to eukaryotic subunit III is lost during purification, SDS-PAGE Dictyostelium discoideum
7.1.1.9 13000
-
x * 55000 + x * 29500 + x * 19000 + x * 13000 + x * 11000 + x * 5700, homolog to eukaryotic subunit III is lost during purification, SDS-PAGE Dictyostelium discoideum
7.1.1.9 19000
-
x * 55000 + x * 29500 + x * 19000 + x * 13000 + x * 11000 + x * 5700, homolog to eukaryotic subunit III is lost during purification, SDS-PAGE Dictyostelium discoideum
7.1.1.9 29500
-
x * 55000 + x * 29500 + x * 19000 + x * 13000 + x * 11000 + x * 5700, homolog to eukaryotic subunit III is lost during purification, SDS-PAGE Dictyostelium discoideum
7.1.1.9 55000
-
x * 55000 + x * 29500 + x * 19000 + x * 13000 + x * 11000 + x * 5700, homolog to eukaryotic subunit III is lost during purification, SDS-PAGE Dictyostelium discoideum

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.9 Dictyostelium discoideum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.9 hydrophobic chromatography, affinity chromatography on horse cytochrome c Dictyostelium discoideum

Storage Stability

EC Number Storage Stability Organism
7.1.1.9 -80°C, 5 mM Tris-cacodylate buffer, pH 7.6 Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.9 ferrocytochrome c + O2 + H+
-
Dictyostelium discoideum ferricytochrome c + H2O
-
r

Subunits

EC Number Subunits Comment Organism
7.1.1.9 ? x * 55000 + x * 29500 + x * 19000 + x * 13000 + x * 11000 + x * 5700, homolog to eukaryotic subunit III is lost during purification, SDS-PAGE Dictyostelium discoideum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.9 158
-
ferrocytochrome c horse cytochrome c Dictyostelium discoideum
7.1.1.9 300
-
ferrocytochrome c
-
Dictyostelium discoideum