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Literature summary extracted from

  • Yamanaka, T.; Fukumori, Y.
    The nitrite oxidizing system of Nitrobacter winogradskyi (1988), FEMS Microbiol. Rev., 54, 259-270.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.1.1.9 cardiolipin stimulation of reaction with horse ferrocytochrome c, reactivity with cytochrome c550 is not affected Nitrobacter winogradskyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.9 0.024
-
 ferrocytochrome c550
-
 Nitrobacter winogradskyi
7.1.1.9 0.11
-
 ferrocytochrome c horse cytochrome c Nitrobacter winogradskyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.9 Cu 2 Cu atoms per enzyme molecule Nitrobacter winogradskyi
7.1.1.9 Mg 1 mol per monomeric functional unit Nitrobacter winogradskyi
7.1.1.9 additional information enzyme does not contain Zn Nitrobacter winogradskyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.9 27000
-
 1 * 40000 + 1 * 27000, SDS-PAGE Nitrobacter winogradskyi
7.1.1.9 40000
-
 1 * 40000 + 1 * 27000, SDS-PAGE Nitrobacter winogradskyi

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.9 Nitrobacter winogradskyi
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.9 ferrocytochrome c + O2 horse ferrocytochrome c Nitrobacter winogradskyi ferricytochrome c + H2O
-
 ?
7.1.1.9 ferrocytochrome c + O2 ferrocytochrome c550 Nitrobacter winogradskyi ferricytochrome c + H2O
-
 ?
7.1.1.9 ferrocytochrome c550 + O2 + H+
-
 Nitrobacter winogradskyi ferricytochrome c550 + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
7.1.1.9 dimer 1 * 40000 + 1 * 27000, SDS-PAGE Nitrobacter winogradskyi

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.9 heme a
-
 Nitrobacter winogradskyi