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Literature summary extracted from

  • Kuhnl, T.; Koch, U.; Heller, W.; Wellmann, E.
    Chlorogenic acid biosynthesis: Characterization of a light-induced microsomal 5-O-(4-coumaroyl)-D-quinate/shikimate 3'-hydroxylase from carrot (Daucus carota L.) cell suspension cultures (1987), Arch. Biochem. Biophys., 258, 226-232.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.96 diethyldithiocarbonate 2fold activation at 0.2-2 mM Daucus carota
1.14.14.96 KCN 2fold activation at 1-10 mM Daucus carota
1.14.14.96 light exposure 4fold activation after 20 h irradiation at 350 nm Daucus carota

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.96 cytochrome c 76% inhibition at 0.1 mM Daucus carota
1.14.14.96 diethyldicarbonate 31% inhibition at 2 mM Daucus carota
1.14.14.96 p-chloromercuribenzoate 32% inhibition at 0.5 mM Daucus carota
1.14.14.96 Tetcyclacis 38% inhibition at 0.01 mM Daucus carota

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.96 microsome
-
 Daucus carota
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.96 trans-5-O-(4-coumaroyl)-D-quinate + [reduced NADPH-hemoprotein reductase] + O2 Daucus carota specific for the trans isomer, final step in the chlorogenic acid pathway, reaction is greatly enhanced by irradiation with blue/uv light trans-5-O-caffeoyl-D-quinate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.96 trans-5-O-(4-coumaroyl)shikimate + [reduced NADPH-hemoprotein reductase] + O2 Daucus carota 70% of the activity compared to trans-5-O-(4-coumaroyl)-D-quinate, specific for the trans isomer trans-5-O-caffeoylshikimate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.96 Daucus carota
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.96 cell culture
-
 Daucus carota
-

Storage Stability

EC Number Storage Stability Organism
1.14.14.96 -70°C, stable for several months Daucus carota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.96 trans-5-O-(4-coumaroyl)-D-quinate + [reduced NADPH-hemoprotein reductase] + O2 specific for the trans isomer, final step in the chlorogenic acid pathway, reaction is greatly enhanced by irradiation with blue/uv light Daucus carota trans-5-O-caffeoyl-D-quinate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.96 trans-5-O-(4-coumaroyl)shikimate + [reduced NADPH-hemoprotein reductase] + O2 70% of the activity compared to trans-5-O-(4-coumaroyl)-D-quinate, specific for the trans isomer Daucus carota trans-5-O-caffeoylshikimate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.96 30
-
 half-life: 10 min Daucus carota

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.96 7.5
-
-
 Daucus carota

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.14.96 6.3
-
 half-maximal activity Daucus carota
1.14.14.96 8.6
-
 half-maximal activity Daucus carota