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Literature summary extracted from

  • Andersson, U.; Levander, F.; Radström, P.
    Trehalose-6-phosphate phosphorylase is part of a novel metabolic pathway for trehalose utilization in Lactococcus lactis (2001), J. Biol. Chem., 276, 42707-42713.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.216 overexpression in Escherichia coli and Lactococcus lactis Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.216 0.9
-
 beta-D-glucose 1-phosphate
-
 Lactococcus lactis
2.4.1.216 4
-
 glucose 6-phosphate
-
 Lactococcus lactis
2.4.1.216 6
-
 trehalose 6-phosphate
-
 Lactococcus lactis
2.4.1.216 32
-
 phosphate
-
 Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.216 94000
-
 gel filtration Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.216 trehalose 6-phosphate + phosphate Lactococcus lactis formation of trehalose 6-phosphate is favoured glucose 6-phosphate + beta-D-glucose 1-phosphate
-
 r
2.4.1.216 trehalose 6-phosphate + phosphate Lactococcus lactis IL1403 formation of trehalose 6-phosphate is favoured glucose 6-phosphate + beta-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.216 Lactococcus lactis Q9CID5 CAA77100
-
2.4.1.216 Lactococcus lactis IL1403 Q9CID5 CAA77100
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.216 to homogeneity Lactococcus lactis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.216 cell culture
-
 Lactococcus lactis
-
2.4.1.216 culture condition:trehalose-grown cell
-
 Lactococcus lactis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.216 32.4
-
-
 Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.216 trehalose 6-phosphate + phosphate formation of trehalose 6-phosphate is favoured Lactococcus lactis glucose 6-phosphate + beta-D-glucose 1-phosphate
-
 r
2.4.1.216 trehalose 6-phosphate + phosphate formation of trehalose 6-phosphate is favoured Lactococcus lactis IL1403 glucose 6-phosphate + beta-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.216 monomer 1 * 94000, SDS-PAGE Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.216 TrePP
-
 Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.216 35
-
-
 Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.216 6.3
-
-
 Lactococcus lactis