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Literature summary extracted from
- Purification and properties of the nitrate reductase isolated from Neurospora crassa mutant nit-3. Kinetics, molecular weight determination, and cytochrome involvement (1974), Biochemistry, 13, 2289-2294.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.7.1.3 | additional information | nit-3 mutant (FGSC 262): reduced FAD-nitrate reductase and reduced methylviologen-nitrate reductase activities | Neurospora crassa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.1.3 | p-hydroxymercuribenzoate | at 0.1 mM complete inhibition | Neurospora crassa |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.7.1.3 | 235000 | - |
sucrose density gradient centrifugation, gel filtration | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.7.1.3 | nitrate + NADPH | Neurospora crassa | - |
nitrite + NADP+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.7.1.3 | Neurospora crassa | - |
wild-type STA4, FGSC 358 and nit-1 mutant, FGSC 262 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.7.1.3 | partially purified | Neurospora crassa |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.7.1.3 | nitrite + NADP+ + H2O = nitrate + NADPH + H+ | sulfhydryl groups may participate in the binding of the protein subunits | Neurospora crassa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.7.1.3 | 8.55 | - |
- |
Neurospora crassa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.7.1.3 | -20°C, stable for 6 months or longer, 5% loss of activity | Neurospora crassa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.7.1.3 | nitrate + NADPH | - |
Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
| 1.7.1.3 | nitrate + NADPH | associated FAD-nitrate reductase and methylviologen-nitrate activity | Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.7.1.3 | More | at least 2 subunits suggested | Neurospora crassa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.7.1.3 | 60 | - |
labile, loss of acitvity after 2 min | Neurospora crassa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.7.1.3 | additional information | - |
optima of associated activities | Neurospora crassa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.1.3 | cytochrome b557 | - |
Neurospora crassa | |
| 1.7.1.3 | FAD | - |
Neurospora crassa | |
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