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Literature summary extracted from

  • Horner, R.D.
    Purification and comparison of nit-1 and wild-type NADPH:nitrate reductases of Neurospora crassa (1983), Biochim. Biophys. Acta, 744, 7-15.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.3 additional information nit-1 mutant, suggested to produce the complete apoenzyme Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.3 0.022 0.05 NADPH
-
 Neurospora crassa
1.7.1.3 0.29
-
 nitrate
-
 Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.3 Molybdenum
-
 Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.3 145000
-
 monodimer, 2 * 145000 Neurospora crassa
1.7.1.3 290000
-
-
 Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.3 nitrate + NADPH Neurospora crassa
-
 nitrite + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.3 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.3 protamine sulfate and ammonium sulfate precipitation, hemoglobin-Sepharose column, Bio-Gel A, FAD-Sepharose affinity column. proteolysis during extensive purification Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.3 0.063
-
 nit-1 mutant Neurospora crassa
1.7.1.3 1.24
-
 wild-type Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
1.7.1.3 -10°C, 0.1 M sodium phosphate buffer, pH 7.3, 0.17 M NaCl, 1 mM dithiothreitol, 5 m M EDTA, 0.5 mM PMSF, 1%ethanol, 30% glycerol Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.3 nitrate + NADPH
-
 Neurospora crassa nitrite + NADP+ + H2O
-
 ?
1.7.1.3 nitrate + NADPH 4 activities: NADPH-nitrate reductase, FADH-nitrate reductase, reduced methyl viologen-nitrate reductase and NADPH-cytochrome c reductase Neurospora crassa nitrite + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.3 dimer monodimer, 2 * 145000 Neurospora crassa
1.7.1.3 More nit-1 mutant enzyme is the apoprotein of nitrate reductase Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.3 25
-
-
 Neurospora crassa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.3 49
-
 50% loss of activity after 1.5 min Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.3 7.3
-
 assay at Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.3 FAD
-
 Neurospora crassa