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Literature summary extracted from

  • Cooley, R.N.; Tomsett, A.B.
    Determination of the subunit size of NADPH-nitrate reductase from Aspergillus nidulans (1985), Biochim. Biophys. Acta, 831, 89-93.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.3 Molybdenum part of cofactor Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.3 91000
-
 2 * 91000, homodimer, SDS-PAGE, smaller bands are caused by proteolytic cleavage Aspergillus nidulans
1.7.1.3 180000
-
-
 Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.3 nitrate + NADPH Aspergillus nidulans
-
 nitrite + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.3 Aspergillus nidulans
-
 biA1, biotin-requiring
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.3 quick purification by immunoprecipitation with monospecific anti-nitrate reductase serum, stabilization during purification and protection of proteolytic cleavage by addition of phenylmethylsulphonyl fluoride Aspergillus nidulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.3 mycelium
-
 Aspergillus nidulans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.3 nitrate + NADPH
-
 Aspergillus nidulans nitrite + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.3 dimer 2 * 91000, homodimer, SDS-PAGE, smaller bands are caused by proteolytic cleavage Aspergillus nidulans